家兔急性肾衰时肾脏的扫描电镜观察

皮下注射50%的甘油生理盐水15ml/kg,复制家兔急性肾功能衰竭的动物模型,用扫描电镜观察了注甘油后1,3,6,24,72h 的肾脏形态学改变。电镜观察结果表明:肾衰初发期,肾小球内皮细胞和上皮细胞均有改变,肾脏微血管中纤维蛋白形成,肾小球内皮细胞和上皮细胞(足细胞)表面以及肾脏微血管内均覆盖无定形颗粒物质;肾衰维持期,除见上述小球和微血管改变更明显外,尚有典型的小管破裂和蛋白管型形成。这提示在家兔甘油性急性肾衰发生的早期,肾小球和无定形颗粒物质的作用可能比肾小管的作用更重要。

A Scanning Electron Microscopic Study on a Rabbit Model with Acute Renal Failure Caused by Glycerol

Acute renal failure was inflicted on 38 rabbits with the subcutaneous injection of 50 per cent glycerin solution 15ml/kg.The renal pathologic changes in the rabbits were observed by using scanning electron microscopy from 1 to 72 hours after the injection of glycerin.The re- sults showed:in the initial phase,both the glomerular endothelium and epithelium changed pathologically and there was fibrin formation in the renal microvascularity.The glomerular endothelium and epithelium were covered by dense amorphous particulate matter through the initial and maintain phase of the acute renal failure.The renal tubules were obstructed by casts,and the tubule rupture was found in the maintain phase.These findings suggested that early in the course of the disease the affects of glomerular changes and accumulation of for- eign matter might be more important than the renal tubule for the pathogenesis of glycerol-induced acute renal failure in rabbits.