国人9号和17号染色体膀胱移行细胞癌相关微卫星位点的杂合性丢失

目的 探讨国人9号和17号染色体微卫星位点的杂合性丢失(LOH)与膀胱移行细胞癌(TCC)发生、发展的关系。方法 选取微卫星位点D9S156、D9S157、D17S786和TP53，对24例TCC组织和外周静脉血提取的DNA用特异引物进行PCR扩增，扩增产物进行6％变性聚丙烯酰胺凝胶电泳和银染，并进行LOH分析。结果 至少有1个位点出现LOH的TCC组织为58．3％(14／24)，同一组织2个位点同时出现LOH的肿瘤为12．5％(3／24)。X^2检验和Fisher精确概率检验(P＞O．05)，4个位点LOH的发生在浅表性和浸润性TCC之间无显著性差异。结论 国人9号染色体上肿瘤抑制基因(TSG)的失活与TCC的发生有关，与TCC的浸润性生长无关。17号染色体上TSG的失活可能与TCC的浸润性生长有关。

Transitional cell carcinoma of urinary bladder related loss of heterozygosity of microsatellite loci on chromosomes 9 and 17 in Chinese

Objective To investigate the relationship between loss of heterozygosity (LOH) of microsatellite loci on chromosomes 9 and 17 in Chinese and development of transitional cell carcinoma (TCC) of urinary bladder. Methods LOH were studied in the tumor tissues of 24 patients, with their peripheral blood as control, by means of PCR amplification of 4 polymorphic microsatellite DNA loci D9S156 and D9S157 on chromosome 9 and D17S786 and TP53 on chromosome 17. Following silver staining, 6% denaturing polyacrilamide gel electrophoresis was performed. Results Fourteen (58 3%) TCCs showed LOH at one or more loci, and 3 TCCs showed LOH at 2 loci. There was no significant difference between superfacial TCC and invasive TCC at these loci. Conclusion LOH on chromosome 9 might be an early event of the genesis of TCC of bladder, and LOH on chromosome 17 might be related with progression of TCC. Racial difference may contribute to different LOH on chromosome 9 and 17 between Chinese people and others.