HPLC法测定天麻头风灵胶囊中原儿茶酸紫丁香苷和绿原酸的含量

目的 建立同时测定天麻头风灵胶囊中原儿茶酸、紫丁香苷、绿原酸含量的方法。方法 采用HPLC法。色谱柱为Diamonsil C18 柱(200 mm×4.6 mm, 5 μm)，流动相为甲醇-乙腈-0.05 %磷酸溶液(体积比20:5:174)，流速为1.0 mL·min-1，柱温为 35 ℃，检测波长为 281 nm。 结果 原儿茶酸、紫丁香苷、绿原酸质量浓度分别在2.40~24.0 mg·L-1、5.20 ~ 52.0 mg·L-1、26.6 ~ 266 mg·L-1内与峰面积呈良好的线性关系，r分别为0.999 6、0.999 7、0.999 5，平均回收率分别为101.0 % (RSD=0.86 % , n= 9)、100.3 % (RSD=1.9 % , n= 9) 、 101.5 % (RSD=1.4 % , n=9)。结论 HPLC法可用于天麻头风灵胶囊的质量控制。

Stimultaneous determination of contents of protocatechuic acid, syringin and chlorogenic acid in Tianma toufengling capsules by HPLC

Objective To develop a HPLC method for the determination of protocatechuic acid, syringin and chlorogenic acid in Tianma toufengling (traditional Chinese medicines) capsules. Methods The chromatographic separation was achieved on a Diamonsil C18 (200 mm×4.6 mm, 5 μm) column with a mobile phase of methanol-acetonitrile-0.05 % phosphoric acid solution (V:V:V=20:5:174). The flow rate was 1.0 mL·min-1 and the column temperature was 35 ℃. Results The calibration curve was linear within the range of 2.40-24.0 mg·L-1 (r=0.999 6) for protocatechuic acid, 5.20-52.0 mg·L-1 (r=0.999 7) for syringin and 26.6-266 mg·L-1 (r=0.999 5) for chlorogenic acid, respectively. The recoveries of protocatechuic acid, syringin and chlorogenic acid were 101.0 % (RSD=0.86 %, n=9), 100.3 % (RSD=1.9 %, n=9) and 101.5 % (RSD=1.4 %, n=9), respectively. Conclusion The method is accurate, sensitive and specific. It can be used for the quality control of Tianma oufengling capsules.