| 胰岛素样生长因子Ⅰ的抗心肌细胞凋亡作用 |
| |
| 引用本文： | 孙贺英,赵荣瑞,支建明. 胰岛素样生长因子Ⅰ的抗心肌细胞凋亡作用[J]. 山西医科大学学报, 2001, 32(Z1): 103-105 |
| |
| 作者姓名： | 孙贺英 赵荣瑞 支建明 |
| |
| 作者单位： | 山西医科大学生理学教研室, |
| |
| 摘 要： | 研究了缺氧条件下引起培养的乳鼠心肌细胞凋亡及IGF－Ⅰ的抗凋亡作用。将原代培养的心肌细胞置于95%N
|
| 关 键 词： | 胰岛素样生长因子I； 心肌； 细胞凋亡； 低氧； DNA片段； 电泳，凝胶 |
| 文章编号： | 1007-6611(2001)S0-0103-03 |
| 修稿时间： | 2000-11-30 |
Effects of insulin-likegrowth factor- |
| |
| SUN He-ying,ZHAO Rong-rui,ZHI Jian-ming. Effects of insulin-likegrowth factor-[J]. Journal of Shanxi Medical University, 2001, 32(Z1): 103-105 |
| |
| Authors: | SUN He-ying ZHAO Rong-rui ZHI Jian-ming |
| |
| Abstract: | The effects of insulin-like growth factor-Ⅰ (IGF-Ⅰ) on cardiomyocyte apoptosis induced by hypoxia in cultured neonatal rat cardiac myocytes were investigated.Primary neonatal rat cardiomyocytes were cul tured in 95% N2－5% CO2 to imitate in vivo hypoxic condition.DNA gel ele ctrophoresis showed DNA laddering in an ischemic duration-dependent manner.The hypoxia-induced cardiomyocyte apoptosis was also evidenced by flow cytometry as say.DNA gel electrophoresis showed that IGF－Ⅰ in a dose range of 10-9～1 0-7mol/L could significantly prevent the hypoxia-induced cardiomyocyte ap optosis.The protective effects of IGF－I against hypoxia-induced apoptosis coul d also be verified by flow cytometry assay. |
| |
| Keywords: | |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
