| 生物降解九资河茯苓多糖的HPLC图谱分析 |
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| 引用本文： | 王知龙,张梦梦,高 帆,叶 晶,吴 鹏.生物降解九资河茯苓多糖的HPLC图谱分析[J].中国酿造,2016,35(7):139. |
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| 作者姓名： | 王知龙 张梦梦 高 帆 叶 晶 吴 鹏 |
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| 作者单位： | 黄冈师范学院生命科学学院，经济林木种质改良与资源综合利用湖北省重点实验室，
大别山特色资源开发湖北省协同创新中心，湖北黄冈438000 |
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| 基金项目： | 湖北省自然科学基金项目（2013CFB472）；校生物示范中心项目（zx201422） |
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| 摘 要： | 通过柱前衍生化高效液相色谱（HPLC）法分析生物降解茯苓多糖的组分。选用黑曲霉HS-5液体发酵，生物降解茯苓多糖。粗多糖液经浓缩、Sevage法纯化、真空干燥处理得到多糖固体样品。利用红外光谱（IR）和紫外光谱（UV）分析鉴定多糖，采用PMP柱前衍生化HPLC法分析，通过与对照品的出峰时间比较，确定其分子中的单糖组成与含量分别为：甘露糖5.463%、鼠李糖8.970%、D-木糖52.809%。
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| 关 键 词： | 茯苓多糖 生物降解 紫外光谱 红外光谱 高效液相色谱 |
HPLC chromatogram analysis of Jiuzihe pachymaran by biodegradation |
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| WANG Zhilong,ZHANG Mengmeng,GAO Fan,YE Jing,WU Peng.HPLC chromatogram analysis of Jiuzihe pachymaran by biodegradation[J].China Brewing,2016,35(7):139. |
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| Authors: | WANG Zhilong ZHANG Mengmeng GAO Fan YE Jing WU Peng |
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| Affiliation: | Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains, Hubei Key Laboratory of
Economic Forest Germplasm Improvement and Resources Comprehensive Utilization, College of Life Sciences, Huanggang Normal University, Huanggang 438000, China |
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| Abstract: | The pachymaran components by biodegradation were analyzed by HPLC with pre-column derivatization. Using Aspergillus niger HS-5 as fermentation strains to conduct liquid-state fermentation, pachymaran was degraded by biodegradation. Pachymaran solid sample was obtained by concentrating crude sugar, sevage purification and vacuum drying. Pachymaran solid sample was analyzed and identified by infrared spectroscopy (IR) and ultraviolet spectroscopy (UV), then using HPLC analysis with PMP column derivatization, by comparison with the peak time of the standard, the monosaccharide composition and contents were determined as follows: mannose 5.463%, rhamnose 8.970%, D-xylose 52.809%. |
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| Keywords: | pachymaran biodegradation ultraviolet spectroscopy infrared spectrum HPLC |
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