桃果实有毛/无毛性状的SCAR标记

 以桃品种‘京玉’和‘美味’的正反交69株F1 群体为试材, 利用RAPD技术扩增出了与桃果实有毛/无毛性状(G/ g) 连锁的2 258 bp的多态性片段, 经克隆、测序后, 根据获得的序列重新设计了两对引物进行SCAR转化。引物对BFP94 /BFP95在有毛和无毛个体中均扩增出了2 258 bp的片段, RAPD显示的多态性消失。利用引物对BFP96 /BFP98成功将RAPD标记转化成了SCAR标记, 并命名为SCP2022258。该标记仅在果实有毛的个体中出现, 与有毛/无毛性状的连锁距离为718 cM, 且扩增稳定, 为桃果实有毛/无毛性状育种的分子标记辅助选择奠定了基础。

SCAR Marker Linked to the Peach/Nectarine in Peach

RAPD technique was used in screen molecular marker linked to peach/nectarine character(G/g) in 69 F_1 hybrids resulting from the intraspecific cross between peach cultivar'Jingyu'and nectarine cultivar'Flavortop'. The OPP20 primer resulted in a reproducible bright polymorphic band of 2 258 bp present in the individuals of peach character and peach cultivar'Jingyu'.The polymorphic band was cloned and sequenced.Four primers were designed based on the sequence to transform into SCAR marker.But primer combination BFP94/BFP95 amplified the same-sized band in all individuals resulting in a loss of the original RAPD polymorphism.The RAPD marker was successfully converted into a SCAR marker with primer combination BFP96/BFP98,which was designated SCP20-2258.The linkage distance is 7.8 cM between the SCP20-2258 and the peach/nectarine character.The SCAR marker will be useful for marker-assisted selection for peach/nectarine character in peach breeding programs.