丹参的冠瘿组织培养和丹参酮的产生

用根癌农杆菌感染丹参无菌苗获得冠瘿组织，除菌后的冠瘿组织在无激素的Ms培养基上生长良好。经高压纸电泳检查，冠瘿组织中含有冠痿碱，证实根癌农杆菌的Ti质粒转化成功。冠瘿组织的生长和丹参酮的积累与基本培养基有关，B5和Ms培养基有利于生长．月增殖倍数分别达到102倍和90倍，而67-V和WP培养基则有利于丹参酮的合成，在培养过程中丹参酮能分泌到培养液中。研究表明用冠瘿组织作为培养系统，生产药用植物有效成分具有良好的开发前景。

Crown Gall Tissue Culture and Production of Tanshinone in Salvia miltiorrhiza

Crown galls were induced by direct infection of sterile seedlings with Agrabacterium tumefaciens C58 and the transformation was proved by opine identification. The crown galls grew well in hormone-free medium. B5 and MS basic medium are good for growth of the crown galls in which crown gall (Strain Ca)increased up to 102 and 90 times in a month,respectively. However,67-V and WP basic medium are good for tanshinone production. It showed that crown galls can be utilized as useful culture system to produce secondary metabolites.