SEARCH FOR HERPES SIMPLEX VIRUS TYPE2（HSV－2）AND HUMAN PAPILLOMAVIRUS（HPV）IN THE NORMAL AND ABNORMAL CERVICAL SAMPLES

ＳＥＡＲＣＨＦＯＲＨＥＲＰＥＳＳＩＭＰＬＥＸＶＩＲＵＳＴＹＰＥ２（ＨＳＶ－２）ＡＮＤＨＵＭＡＮＰＡＰＩＬＬＯＭＡＶＩＲＵＳ（ＨＰＶ）ＩＮＴＨＥＮＯＲＭＡＬＡＮＤＡＢＮＯＲＭＡＬＣＥＲＶＩＣＡＬＳＡＭＰＬＥＳＺｈａｎｇＷｅｉ;张伟;ＪｉｎＳｈｕｎｑｉａ...

Search for herpes simplex virus type 2 (HSV-2) and human papillomavirus (HPV) in the normal and abnormal cervical samples

The specimens of 111 cervical carcinomas, 68 chronic cervicitis and 43 normal cervical exfoliated epithelial cells were examined for the presence of HSV-2 DNA sequences with DNA hybridization using HSV-2 BgL I N fragment probe labelled by 32P-dCTP. The result showed that the infection rates of HSV-2 in the samples of cervical cancer, chronic cervicitis and normal epithelial cells were 14.41% (16/111), 27.94% (19/68) and 25.58% (11/43), respectively. It was implied that early stages carcinogenesis of cervical epithelial cells might be correlated with the HSV-2 infection. Sixteen HSV- 2 positive samples of cervical carcinomas were also examined for the presence of the sequences homologous to human papillomavirus (HPV) type 6B/11, 16 and 18 DNA using dot blot hybridization (Tm-17C). The result indicated that 13 out of 16 were HPV 16 DNA hybridization positive accounting for 81.2% of all HSV-2 positive samples and none of them were positive for HPV type 6B/11 and 18. The result indicated that double infection of HSV-2 and HPV16 in the same cervical carcinoma tissues may provide a strong evidence of the viral synergistic interaction in the induction of female cervical carcinomas.