A CO2-enriched atmosphere improves in vitro growth of Brazilian ginseng [Pfaffia glomerata (Spreng.) Pedersen] |
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Authors: | Cleber Witt Saldanha Caio Gomide Otoni Marcela Morato Notini Kacilda Naomi Kuki Ana Cláudia Ferreira da Cruz Aurélio Rubio Neto Leonardo Lucas Carnevalli Dias Wagner Campos Otoni |
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Affiliation: | 1. Laboratório de Cultura de Tecidos (LCTII)/BIOAGRO, Departamento de Biologia Vegetal, Universidade Federal de Vicosa, Vicosa, 36570-000, MG, Brazil 2. Laboratório de Embalagens (LABEM), Departamento de Tecnologia de Alimentos, Universidade Federal de Vicosa, Vicosa, 36570-000, MG, Brazil 3. Laboratório de Cultura de Tecidos, Departamento de Fitotecnia, Universidade Federal de Vi?osa, Vicosa, 36570-000, MG, Brazil 4. Plant Biology Department, Plant Tissue Culture Laboratory/BIOAGRO, University Campus, Peter Henry Rolfs Avenue, Federal University of Vi?osa, Vicosa, 36570-000, MG, Brazil
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Abstract: | The aim of the present study was to evaluate the effects of forced ventilation and CO2 enrichment (360 or 720 μmol mol?1 CO2) on the in vitro growth and development of Pfaffia glomerata, an endangered medicinal species, under photomixotrophic or photoautotrophic conditions. P. glomerata nodal segments showed substantial differences in growth, relative water content and water loss from leaves, photosynthetic pigments, stomatal density, and leaf anatomical characteristics under these different treatments. CO2 enrichment led to increased photosynthetic pigments and reduced stomatal density of in vitro cultivated P. glomerata. A lack of sucrose in the culture medium increased 20-hydroxyecdysone levels, but the increase in CO2 levels did not further elevate the accumulation of 20-hydroxyecdysone. All growth increased in a CO2-enriched atmosphere. In addition, CO2 enrichment, with or without sucrose, gave a lower relative water loss from leaves. This finding indicates that either a photoautotrophic or photomixotrophic system in a CO2-enriched atmosphere may be suitable for large-scale propagation of this species. |
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