中国内陆6省(自治区)淡水鱼养殖、销售和餐饮环节常见嗜盐性弧菌污染调查

目的了解内陆6省(自治区)淡水鱼养殖、销售、餐饮环节鱼、水体、水底沉积物等样品中副溶血性弧菌、溶藻弧菌、创伤弧菌的污染状况,明确淡水鱼养殖、销售、餐饮各环节中3种嗜盐性弧菌的分布。方法在广西、河南、湖北、山东、四川、浙江6个省(自治区)的内陆地市设置采样点,采集养殖场、销售场所、餐馆的鱼样及存养鱼的对应水样。采用传统的分离培养技术检测3种嗜盐性弧菌,并进行生化鉴定。结果养殖环节水体和淡水鱼中副溶血性弧菌的检出率分别为1.89%(5/264)、5.81%(14/241),溶藻弧菌检出率分别为1.89%(5/264)、4.98%(12/241),创伤弧菌检出率分别为1.14%(3/264)、0.83%(2/241);3种嗜盐性弧菌的污染率在流通环节增高,存养水体和淡水鱼的检出率分别为副溶血性弧菌18.47%(29/157)、14.93%(40/268),溶藻弧菌10.83%(17/157)、9.70%(26/268),创伤弧菌3.18%(5/157)、3.36%(9/268);餐饮环节淡水鱼中嗜盐性弧菌的检出率与流通环节基本一致。结论传统意义上海水中常见的副溶血性弧菌、创伤弧菌、溶藻弧菌3种嗜盐性弧菌已经在内陆地区的淡水养殖、流通和餐饮各个环节中检出,尤其是淡水养殖环境中嗜盐性弧菌的出现可能对整个食物链产生的影响值得关注。

Monitoring of halophilic Vibrio spp. from farming, saling and catering of freshwater fish in inland cities

To study the contamination and distribution of Vibrio parahemolyticus, Vibrio alginolyticu and Vibrio vulnificus in fish, water and underwater deposit from farming, saling and catering of freshwater fish in inland cities in China.Methods All monitoring sites were in Guangxi, Henan, Hubei, Shandong, Sichuan and Zhejiang Provinces. Three kinds of halophilic Vibrio from different samples were analyzed by conventional method such as culturing, isolating and biochemical identification. Results The occurrence was 1.89% and 5.81% for Vibrio parahemolyticus isolated from farming water and freshwater fish, 1.89% and 4.98% for Vibrio alginolyticu, and 1.14% and 0.83% for Vibrio vulnificus. The occurrence increased rapidly in markets from temporary keeping water and freshwater fish with 18.47% and 14.93% for Vibrio parahemolyticus, 10.83% and 9.70% for Vibrio alginolyticu, and 3.18% and 3.36% for Vibrio vulnificus. The occurrence of three kinds of halophilic Vibrio isolated from freshwater fish was basically identical between markets and the restaurants.Conclusion The marine Vibrio, Vibrio parahemolyticus, Vibrio alginolyticu andVibrio vulnificus, have been isolated from farming, saling and catering of freshwater fish in inland cities, and it should call attention to the safety of the food chain.