| 人神经干细胞端粒酶活性检测 |
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| 引用本文： | 刘学强,杨辉,何家全,宋业纯,吕胜青,刘仕勇. 人神经干细胞端粒酶活性检测[J]. 中国微侵袭神经外科杂志, 2005, 10(6): 260-262 |
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| 作者姓名： | 刘学强 杨辉 何家全 宋业纯 吕胜青 刘仕勇 |
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| 作者单位： | 中国人民解放军第三军医大学新桥医院神经外科,重庆,400037 |
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| 基金项目： | 国家自然科学基金资助项目(30130110) |
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| 摘 要： | 目的检测体外培养的人神经干细胞端粒酶活性,分析其与神经干细胞生物学特性的关系。方法分离、鉴定并扩增取自10~14周药物流产胎儿大脑皮质的神经干细胞,应用PCR-ELISA法检测神经干细胞端粒酶活性。结果经免疫组化检查,培养的细胞为人神经干细胞;其在体外培养过程中,仅在早期10代表达极低的端粒酶活性(相对活性为6.4%~21.2%),其后消失。结论神经干细胞表达端粒酶活性,但较低且不能长期维持,这可能是神经干细胞不能长期传代的原因之一。
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| 关 键 词： | 干细胞 端粒 末端转移酶 |
| 文章编号： | 1009-122X(2005)06-0260-03 |
| 修稿时间： | 2005-03-09 |
Detection of telomerase activity in human neural stem cells |
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| LIU Xueqiang,YANG Hui,HE Jiaquan,et al. Detection of telomerase activity in human neural stem cells[J]. Chinese Journal of Minimally Invasive Neurosurgery, 2005, 10(6): 260-262 |
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| Authors: | LIU Xueqiang YANG Hui HE Jiaquan et al |
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| Affiliation: | LIU Xueqiang,YANG Hui,HE Jiaquan,et alDepartment of Neurosurgery,Xinqiao Hospital,Third Military Medical University of PLA,Chongqing 400037,China |
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| Abstract: | Objective To detect the telomerase activity in human neural stem cells, and analyze the relation between telomerase activity and the biological characteristics of human neural stem cells. Methods The neural stem cells were isolated, identified and amplified from the cortex of aborted human embryo with 10 to 14-week gestational age, then telomerase activity was detected by PCR-ELISA. Results Immunohistochemical test showed that the cultured cells were neural stem cells. At early 10 passage human cultured neural stem cells expressed very low levels of telomerase with the relative activity of 6.4% to 21.2%, and disappeared whereafter. Conclusion Human neural stem cells express low level of telomerase activity, and could not maintain for long time. This may be the one of the reasons that human neural stem cells could not be long-term passaged in vitro. |
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| Keywords: | stem cells telomerase |
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