慢性阻塞性肺疾病中MMP-9/TIMP-1与Th17/Treg作用与联系

目的 检测慢性阻塞性肺疾病（COPD）外周血中Th17、Treg细胞占CD4^＋T细胞的比例、Th 17/Treg比值和血浆中MMP-9、TIMP-1含量、MMP-9/TIMP-1比值,探讨其在发病中的作用和机制.方法 COPD急性加重组患者30例,COPD缓解组25例患者健康吸烟组20例患者,健康非吸烟组20例患者;采用流式细胞分析技术检测Th17、Treg细胞占CD4^＋T细胞的比例,ELISA法检测血浆中MMP-9、TIMP-1含量.结果 外周血Th17占CD4^＋T细胞的比例在：COPD急性期组高于缓解组、健康非吸烟组、健康吸烟组,差异有统计学意义（P＜0.05）,COPD缓解期组与健康吸烟组相比差异无统计学意义（P＞0.05）,与健康非吸烟组相比差异有统计学意义（P＜0.05）.外周血Treg占CD4＋T细胞比例在：各组间比较,差异无统计学意义（F=1.971,P＞0.05）;Th17/Treg比值的：COPD急性期组分别与COPD缓解期组、健康吸烟组、健康非吸烟组比较,差异均有统计学意义（P＜0.05）,COPD缓解期组与健康吸烟和健康非吸烟组比较,差异无统计学意义（P＞0.05）;血浆中MMP-9含量的在：COPD急性期组与COPD缓解期组、健康吸烟组、健康非吸烟组比较,差异均有统计学意义（P＜0.05）,COPD缓解期组与健康吸烟组、健康非吸烟组比较,差异有统计学意义（P＜0.05）,血浆中MMP-9/TIMP-1比值：COPD急性加重期组与其他三组相比均有统计学意义（P＜0.05）,COPD缓解期组与健康组相比,均有统计学意义（P＜0.05）.结论 Th17、Treg细胞、MMP-9、TIMP-1可能均参与了COPD的发生和发展.Th17、MMP-9增高可能促发COPD的发生和急性加重,并且两者存在一定相关性,并相互影响,加重COPD的自体免疫反应.

Roles and correlations of Th17/Treg and MMP-9/TIMP-1 in chronic obstructive pulmonary disease

Objective To Detect Th17/CD4^＋ （ % ） and Treg/CD4 ^＋ （ % ） in peripheral blood and the ratio of Th17/Treg and the amount of MMP-9 and TIMP-1 in plasma, MMP-9/TIMP-1 in patients with chronic obstructive pulmonary disease （COPD） , and to analyze their effects in the pathogenesis of COPD. Methods Th17/CD4 ^＋ （ % ） and Treg/CD4 ^＋ （ % ） were detected by flow cytometry, the levels of MMP-9 and TIMP-1 in the plasma were detected by ELISA method. Results Th17/CD4 ^＋ T （ % ） ： COPD acute group was higher than COPD remission group, healthy nonsmokers group and healthy smokers group, with the statistic difference was significant （P 〈 0.05 ）. COPD remission group compare with healthy smokers, the statistic difference was not significant（ P 〉 0.05 ）. While COPD remission group compare with healthy non-smoking group, the statistic difference was significant （ P 〈 0.05 ）. Treg/CD4^＋ T（ % ） ： the difference was not statistically significant across groups （F = 1. 971, P 〉0. 05）. Th17/Treg （%）： COPD acute group respectively compare with COPD remission group, healthy smokers and healthy nonsmokers, the statistic differences were all significant （P 〈 0. 05 ）. COPD remission group compare with healthy non-smokers group and healthy smokers group, the difference was not statistically significant （ P 〉 0.05 ）. Levels of MMP-9 in plasma ： COPD acute group respectively compare with COPD remission group, healthy smokers group and healthy non-smokers group, and the statistic differences were all significant （ P 〈 0.05 ） ; COPD remission group Compare with the healthy nonsmokers group and healthy smokers group, both of the statistic differences were significant （ P 〈 0.05 ）. MMP-9/TIMP-1 （%）： COPD acute group respectively compare with the COPD remission group, healthy nonsmokers group and healthy smokers group, the statistic differences were all significant （ P 〈 0.05 ） ; COPD remission group compare with healthy groups, both of t