Countercurrent approach to the enrichment of Δ9c,11t-and Δ10t,12c-18:2 isomers by urea complexation |
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Authors: | David W L Ma Catherine J Field M T Clandinin |
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Affiliation: | (1) Nutrition and Metabolism Research Group, University of Alberta, T6G 2P5 Edmonton, Alberta, Canada;(2) Department of Medicine, Food and Nutritional Science, University of Alberta, T6G 2P5 Edmonton, Alberta, Canada;(3) Department of Agricultural, Food and Nutritional Science, University of Alberta, T6G 2P5 Edmonton, Alberta, Canada;(4) 4-10 Agriculture/Forestry Centre, University of Alberta, T6G 2P5 Edmonton, Alberta, Canada |
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Abstract: | CLA refers to a group of geometrical and positional isomers of linoleic acid. CLA has been shown to have potentially beneficial
effects on cancer, atherosclerosis, and body metabolism in animals. Mixtures containing equal amounts of these isomers are
commonly used in many research studies because of their greater availability and lower cost relative to pure isomers. This
has hindered progress in elucidating the biological properties of specific isomers and their relevance in animal and human
biology. A method was developed that offers a compromise between cost and utility to make available enriched mixtures of either
the Δ9c,11t- or Δ10t,12c-18:2 isomers for use in a wide range of experimental applications. A countercurrent approach was developed to separate the
Δ9c,11t- and Δ10t,12c-18:2 isomers from an equal mixture of these two isomers by urea complexation. After three successive rounds of complexation
using an equal amount of CLA and urea, a fraction enriched in Δ9c,11t-18:2 containing 42.5 and 17.4% of Δ9c,11t-and Δ10t,12c-18:2, respectively, was recovered. After a single round of complexation using 2.5 g urea/g CLA, a fraction enriched in Δ10t,12c-18:2 was recovered containing 29.7 and 69.1% of Δ9c,11t- and Δ10t,12c-18:2, respectively. |
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Keywords: | Conjugated linoleic acid countercurrent purification urea complexation |
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