| 高效液相色谱法测定紫丁香叶中酪醇的含量 |
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| 引用本文： | 裴香萍,张淑蓉,裴妙荣,黄炫,黄灿林. 高效液相色谱法测定紫丁香叶中酪醇的含量[J]. 光明中医, 2008, 23(6): 733-734 |
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| 作者姓名： | 裴香萍 张淑蓉 裴妙荣 黄炫 黄灿林 |
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| 作者单位： | 山西中医学院中药系,太原,030024;山西中医学院中药系,太原,030024;山西中医学院中药系,太原,030024;山西中医学院中药系,太原,030024;山西中医学院中药系,太原,030024 |
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| 摘 要： | 目的：高效液相色谱法测定紫丁香叶中酪醇的含量。方法：采用HPLC法测定紫丁香叶中酪醇的含量。色谱柱：SymmetryC18柱（4．6mm×250mm，10μm）；流动相：甲醇-0．125％冰醋酸溶液（10：90）；流速：1．0ml／min；检测波长：275nm；柱温：室温。结果：酪醇在0．44—3．52μg范围内线性关系良好，回归方程为方程Y=616731x-9957．4，r=0．9993；平均回收率为97．83％。结论：该方法准确可靠，适用于紫丁香叶酪醇的含量测定。
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| 关 键 词： | 紫丁香叶 HPLC 酪醇 |
| 修稿时间： | 2008-03-04 |
To Establish a Method for the Determination of Tyrosol in Syring oblat A Lindl Leaves |
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| Pei Xiangping,Zhang Shurong,Pei Miaorong,et al. To Establish a Method for the Determination of Tyrosol in Syring oblat A Lindl Leaves[J]. Journal of Traditional Chinese Medicine, 2008, 23(6): 733-734 |
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| Authors: | Pei Xiangping Zhang Shurong Pei Miaorong et al |
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| Abstract: | Objective ： To establish a method for the determination of tyrosol in Syringa oblatA Lindl. Leaves. Methods ： HPLC was used on Symmetry C18 column （4.6mm × 250mm, 10um） ; with methand-aceticacid 0. 125% （10：90） as a mobile phase and 1 .0ml/min of flow rate. The detection wavelength was 275nm, and column temperature was room temperature. Results： The calibration curve of tyrosol was linear at the range of 0.44-3.52ug.The regression equation was y = 616731x -9957.4, r = 0.9993. The average recoveries were 98.01%. Condution ： The method is highly accurate and reliable, and is suitable for the determination of tyrosol in Syringa oblata Lindl. Leaves. |
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| Keywords: | Syringa oblata Lindl HPLC tyrosol |
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