脱水对万寿竹种子发芽及部分抗逆生理指标的影响

目的]探究万寿竹种子脱水过程中,发芽及部分抗逆生理指标的变化情况,为万寿竹种子贮藏及采收提供理论依据.方法]利用室温硅胶干燥法获得45.90％、40.50％、38.07％、33.08％、27.26％、16.67％、14.63％7个含水量梯度的种子,以未干燥的种子(49.12％)为空白对照,分别测定各个含水量梯度种子的生活力、发芽率、电导率、CAT、SOD、POD、AsA-POD活性、MDA、可溶性蛋白质含量.结果]种子含水量保持在33.08％以上时,能保持较高的生活力、发芽率,当含水量下降到33.08％以下时,种子发芽率和生活力开始显著下降.结论]万寿竹种子不耐脱水,属于顽拗性种子,贮藏时含水量应保持在33.08％以上.

The Effect of Dehydration on Germination Percentage and Some Indexes of Resistance of Disporum cantoniense Seeds

Objective]In order to provide theoretical basis for Disporum cantoniense seeds storage and harvesting,exploring the change of germination percentage and some indexes of resistance during the period of seed dehydration.Method]The seeds of Disporum cantoniense were dried with silica gel to reduce the moisture content from 49.12％ to 45.90％,40.50％,38.07％,33.08％,27.26％,16.67％ and 14.63％respectively under the room temperature.With the seeds in 49.12％ water content as a blank control group,the seeds should be determined by different indexes,such as viability,germination percentage,conductivity,CAT,SOD,POD,AsA-POD,MDA and soluble protein content.Result]Those seeds with moisture contents above 33.08％ could remained high germination percentage and viability,the viability and germination percentage significantly decreased when seed moisture content was reduced below 44.08％.Conclusion]The Disporum cantoniense seeds have weak desiccation-tolerance,so the seeds belong to recalcitrant seeds.The water content should be keep at above 33.08％ during seed storage period.