土贝母组织培养及植株再生

用土贝母茎段和叶片作外植体培养,可获得大量的试管苗。茎段愈伤组织诱导的最佳培养基为MS 2,4-D 2.0 mg/L NAA 0.5 mg/L BA 1.0 mg/L(单位下同);叶片愈伤组织诱导的最佳培养基配方为MS 2,4-D0.5 NAA 2.0。叶片接入MS BA 3.0 NAA 1.0培养基可直接产生不定芽;愈伤组织用MS BA 2.0 IAA 1.0培养基也可产生不定芽。MS BA 2.0 IAA 0.1适于腋芽发育、增殖。壮苗生根培养基为1/2 MS IBA 1.0。诱导愈伤组织和不定芽,选用pH 6.0,琼脂0.8%~1.0%;诱导生根选用pH 5.8,琼脂0.6%~0.7%。经炼苗后,组培苗移栽成活率达90%。

Tissue Culture and Plant Regeneration of Bolbostemma paniculatum

The tissue culture and plant regeneration of Bolbostemma paniculatum were studied and a large number of regenerated plantlets were obtained.The optimal compounding of medium that induced calli from stems and leaves were the MS medium supplemented with 2,4-D 2.0 mg/L,NAA 0.5 mg/L and BA 1.0 mg/L and the MS medium with 2,4-D 0.5 mg/L and NAA 2.0 mg/L,respectively.Numerous shoots could formed directly when leaf explants were cultured on the MS medium with BA 3.0 mg/L and NAA 1.0 mg/L and calli were cultured on the MS medium with BA 2.0 mg/L and IAA 1.0 mg/L.The MS medium supplemented with BA 2.0 mg/L,IAA 0.1 mg/L was optimal for shoots growth.For the root growth was 1/2MS medium with 1.0 mg/L IBA.At pH 6.0 and 0.8%-1.0% agar was optimal for callus and shoot formation,while pH 5.8 and 0.6%-0.7% agar was optimal for root formation.The tube seedling can be successfully transplanted.