抱茎苦荬菜药材中木犀草素-7-O-β-D-吡喃葡萄糖苷的分离与含量测定

目的建立分离、测定抱茎苦荬菜药材中木犀草素7OβD葡萄糖苷的方法。方法采用醇提取、氯仿萃取与硅胶柱分离等手段来分离木犀草素7OβD葡萄糖苷;采用RP HPLC法进行含量测定,色谱条件为:Diamonsin C18(250 mm×4.6 mm,5μm)色谱柱,以质量分数为0.2%的磷酸溶液甲醇(体积比为67∶33)为流动相,检测波长为260 nm,流速为0.8 mL.min-1。结果木犀草素7OβD葡萄糖苷质量浓度在4.80~24.0 mg.L-1内线性关系良好(r=0.999 7,n=5),平均回收率为99.4%,相对标准差(RSD)为0.97%。结论本法适合测定抱茎苦荬菜药材中木犀草素7OβD葡萄糖苷的含量。

Separation and quantitative determination of luteolin-7-O-β-D-glucopyranoside in the Ixeris sonchifolia (Bge.) Hance

Objective To develop a RP-HPLC method for the determination of the content of luteolin-7-O-β-D-glucopyranoside in Ixeris sonchifolia(Bge.)Hance.Methods The luteolin-7-O-β-D-glucopyranoside was obtained by the extraction with 70%EtOH and precipitated by the method of column chromatography.It was separated on a Diamonsin C_(18)(4.6 mm×250 mm,5 μm)column with CH_3OH-H_2O(0.2%H_3PO_4)(33∶67)as the mobile phase,The UV detective wavelength was set at 260 nm,the flow rate was 0.8 ml/min.Results The average recovery is 99.4%,and RSD is 0.97%.The linear calibration curves are obtained in the ranges of 4.8～24.0 mg·L~(-1).Conclusions The method is simple and accurate.It can be applied to the determination of the concentration of the luteolin-7-O-β-D-glucopyranoside.