Increased level of apolipoprotein B mRNA in the liver of ventromedial hypothalamus lesioned obese rats |
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| Authors: | Y Inui S Kawata Y Matsuzawa K Tokunaga S Fujioka S Tamura T Kobatake Y Keno S Tarui |
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| Affiliation: | Second Department of Internal Medicine, Osaka University Medical School, Japan. |
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| Abstract: | The mRNA level of apolipoprotein B (apoB), which is a principal protein component of nascent very low density lipoprotein (VLDL), was determined in parallel with the measurement of acetyl-coenzyme A (Ac-CoA) carboxylase activity in the liver of ventromedial hypothalamus (VMH) lesioned obese rats. Eight weeks after the electrolysis of the bilateral VMH, the level of apoB mRNA in the VMH-lesioned rats was about 1.5-fold higher than that in the sham-operated rats, indicating increased apoB synthesis in the liver of the VMH-lesioned obese rats. The activity of Ac-CoA carboxylase, which is a rate-limiting enzyme for the fatty acid biosynthesis, was about 1.8-fold higher in the VMH-lesioned rats. These observations indicated that VLDL synthesis is increased in the liver of VMH-lesioned obese rats. |
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