以酚红为底物的伏安酶联免疫分析体系测定HRP

提出酚红 H2 O2 辣根过氧化物酶 (HRP)伏安酶联免疫分析体系。酚红具有醌式结构 ,在滴汞电极上于 - 0 62V(vs .SCE)左右产生伏安还原峰。HRP催化H2 O2 氧化酚红生成非电活性产物 ,使酚红的峰高降低。利用酚红的伏安还原峰的峰高降低 ,可以测定HRP及其标记物 ,并进而可用于酶联免疫分析。以线性扫描二阶导数伏安技术测定HRP的活性 ,其线性范围为 1 .0× 1 0 - 6 ～ 1 .0× 1 0 - 4 g·L- 1 HRP ,检测限为 7.3× 1 0 - 7g·L- 1 HRP。并将该体系应用于烟草花叶病毒的测定。

Voltammetric Enzyme-linked Immunoassay for Dectection of Horseradish Peroxidase Using Phenol Red as Substrate

A new voltammetric enzyme immunoassay based on phenol red H 2 O 2 horseradish peroxidase (HRP) was proposed. Phenol red is an electroactive compound and has a sensitive voltammetric reductive peak at -0.62V (vs.SCE) on the mercury electrode. HRP can catalyze the oxidation reaction of H 2 O 2 with phenol red to produce an electro inactive product, and the reductive peak of phenol red decreased correspondingly, which can be used for the determination of HRP and labeled HRP. The activity of HRP was linear with the decrease of the peak value in the range of 1.0×10 -6 ～1.0×10 -4 g·L -1 with the detection limit of 7.3×10 -7 g·L -1 .This new voltammetric enzyme immunoassay system was applied to the detection of tobacco mosaic virus (TMV).