Specific cell surface labels in the visual centers of Xenopus laevis tadpole identified using monoclonal antibodies |
| |
| Authors: | S Takagi T Tsuji T Amagai T Takamatsu H Fujisawa |
| |
| Affiliation: | 1. Department of Surgery, Kurume University School of Medicine, Fukuoka, Japan;2. Department of Gastroenterology, Kurume University School of Medicine, Fukuoka, Japan;3. Department of Pharmacology, Faculty of Medicine, Osaka Medical College, Osaka, Japan;4. Department of Regenerative Medicine Science, Tokai University School of Medicine, Kanagawa, Japan;5. Group of Vascular Regeneration Research, Institute of Biomedical Research and Innovation, Kobe, Japan;6. RIKEN Center for Developmental Biology, Kobe, Japan;1. College of Chemistry and Chemical Engineering, Tianjin University of Technology, Tianjin 300384, China;2. Tianjin Key Laboratory of Organic Solar Cells and Photochemical Conversion, Tianjin University of Technology, Tianjin 300384, China;1. Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294;2. Department of Pathology, New York University Medical Center, New York, NY 10016;3. Department of Pathology, Southern California Permanente Medical Group, Woodland Hills, CA 91367;4. Department of Pathology, University of Calgary, Alberta, Canada T2L2K8 |
| |
| Abstract: | Monoclonal antibodies (MAbs) against the optic tectum of Xenopus tadpoles were generated and screened by the immunofluorescent staining of frozen sections of tadpole brains. MAb-A5 stains the 8th and 9th plexiform layers of the optic tectum, whereas MAb-B2 stains all but the eighth and ninth plexiform layers of the optic tectum. MAb-A5 antigen is also detectable in the nucleus of Belonci, the corpus geniculatum thalamicum, the pretectal area, and the basal optic nucleus, all targets of the optic nerve, but is not detectable in the optic nerve or the optic tract. On the other hand, MAb-B2 does not stain any of these visual centers, though many fibers surrounding them are stained. Eye-enucleation experiments showed that MAb-A5 antigen is expressed in the optic tectum even when it is not innervated by optic nerves. Staining of viable brains with these MAbs indicates that these antigens are cell surface molecules. Immunoadsorption followed by SDS-PAGE suggests that proteins are constituents of these antigens. The MAb-A5 antigen in the diencephalon and the mesencephalon is not detectable at stage 35/36, but is detectable at stage 39 when the optic nerves begin to innervate the optic tectum. The spatial as well as the temporal patterns of the expression of the MAb-A5 antigen suggest that this molecule may be involved in the target recognition of optic nerve fibers. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
