LHRH-PE40对荷瘤BALB/c小鼠抗肿瘤效果的实验研究

目的 观察LHRH-PE40对小鼠腹水瘤及实体瘤的治疗效果,为确定药理、毒理学实验剂量提供依据。方法 分别给BALB/c小鼠腹腔内和背部皮下接种骨髓瘤SP2/0细胞,接种后第4d和第9d,分别腹腔注射LHRH-PE40,并设空白对照组,停药后第5d处死,观察两组小鼠腹水和腹腔内肿瘤生长情况,称量瘤重,计算抑瘤率。结果 腹水瘤实验组所有小鼠腹部未见腹水,腹腔未见肿瘤。实体瘤实验组抑瘤率为54％。结论LHRH-PE40可以有效地抑制荷瘤小鼠实体瘤的生长。

Antitumor Effect of LHRH-PE40 on Tumor-bearing BALB/c Mouse Model

Objective To observe the curative effect of LHRH - PE40 on ascitic tumor and solid tumor in BALB/c mouse model and provide a basis for the determination of dosages in pharmacological and toxi-cological tests.Methods Inoculate i. d. SP 2/0 cells into BALB/c mice, and inject with LHRH - PE40 4 days after inoculation, once a day for 8 days, using physiological saline as control. Meanwhile, inoculate s. c. SP 2/0 cells into BALB/c mice, and inject with LHRH - PE40 9 days after inoculation, every other day for 8 times, using physiological saline as control. Kill the mice 5 days after the last injection and observe the ascite and the growth of tumors in abdominal cavity. Weigh the tumors and calculate tumor inhibition rate. Results No ascite or tumors was observed in the mice inoculated i. d. with SP 2/0 cells. The tumor inhibition rate of the mice inoculated s. c. with SP 2/0 cells was 54% . Conclusion LHRH - PE40 can inhibit the growth of solid tumors in tumor-bearing mice.