5个大鳞副泥鳅家系的遗传结构分析

利用20个微卫星标记对5个大鳞副泥鳅(Paramisgurnus dabryanus)家系的遗传结构进行分析,探究家系遗传多样性及其亲缘关系的远近,为进一步遗传选育提供技术参数。结果显示,20个微卫星标记在5个家系中共检测到等位基因数53个,平均等位基因数2.65个,各位点的等位基因数2~4个;5个家系平均观测杂合度(Ho)0.3117~0.5542,平均期望杂合度(He)0.3261~0.4371,平均多态信息含量(PIC)0.3459~0.4538,平均固定系数(Fis)-0.0472(<0),遗传分化系数(Fst)0.2703;UPGMA聚类分析显示,AB2013F-7和AB2013F-81亲缘关系最远。研究表明,5个家系间遗传分化较大,具备较大的选育空间。

Genetic Structure Analysis of Five Families of Paramisgurnus dabryanus

Paramisgurnus dabryanus is one of Chinese famous cultured fish, breeding work is still in its infancy. To provide technical parameters for breeding of P. dabryanus, 20 microsatellite markers were applied to analysis of the genetic diversity and kinship distance of of five families. Results showed that a total of 53 alleles were detected in five families of P. dabryanus for 20 SSRs, the mean number of alleles was 2.65, the number ofalleles ranged from 2 to 4 for each loci; the average observed heterozygosity (Ho) ranged from 0.3117 to 0.5542 in five families; the average expected heterozygosity (He) ranged from 0.3261 to 0.4371; the average polymorphic information content (PIC) ranged from 0.3459 to 0.4538; theaverage fixed coefficient(Fis) was -0.0472(<0) and the genetic differentiation coefficient (Fst) was 0.2703 in five families; UPGMA cluster analysis showed the families kinship of AB2013F-7 and AB2013F-81 was the farthest. Studies suggested that five Paramisgurnus dabryanus families had large genetic differentiation and large breeding potential.