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| RP-HPLC法测定中药茯苓皮中茯苓酸A和3-表去氢土莫酸的含量 | |
| 引用本文： | 夏烨,杨春华,刘静涵,濮社班.RP-HPLC法测定中药茯苓皮中茯苓酸A和3-表去氢土莫酸的含量[J].药学进展,2009,33(6):271-273. |
| 作者姓名： | 夏烨 杨春华 刘静涵 濮社班 |
| 作者单位： | 1. 中国药科大学生药学教研室,江苏,南京,210038 2. 中国药科大学分析测试中心,江苏,南京,210009;中国药科大学教育部药物质量与安全预警重点实验室,江苏,南京,210009 3. 中国药科大学天然药物化学教研室,江苏,南京,210009 |
| 摘 要： | 目的：建立一种可用于评价茯苓皮药材质量的含量测定方法。方法：采用Dikma Dimonsal C18色谱柱（250mm×4.6mm，5μm），以0.1％甲酸水溶液和乙腈为流动相，流速：1.0mL·min^-1，检测波长：243nm，柱温：25℃，用梯度洗脱法同时测定茯苓皮药材中茯苓酸A和3-表去氢土莫酸的含量。结果：测得茯苓酸A和3.表去氢土莫酸的线性范围分别为0.073-0.292g·L^-1（r=0.9997）和0.027—0.135g·L^-1（r=0.9999）；加样回收率分别为102.63％和98.88％。结论：该法能快速、准确地测定茯苓皮中两个活性成分的含量，为茯苓皮的质量控制提供了一个较好的检测方法。 |
| 关 键 词： | 茯苓皮 茯苓酸A 3-表去氢土莫酸 反相高效液相色谱 |
Simultaneous Determination of Poricoic Acid A and 3-Epidehydrotumulosic Acid in Poria Peel by RP-HPLC |
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| XIA Ye,YANG Chun-hua,LIU Jing-han,PU She-ban.Simultaneous Determination of Poricoic Acid A and 3-Epidehydrotumulosic Acid in Poria Peel by RP-HPLC[J].Progress in Pharmaceutical Sciences,2009,33(6):271-273. | |
| Authors: | XIA Ye YANG Chun-hua LIU Jing-han PU She-ban |
| Affiliation: | XIA Ye, YANG Chun-hua, LIU Jing-han, PU She-ban(1. Department of Pharmacognosy, China Pharmaceutical University, Nanjing 210038, China; 2. Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China; 3. Center for Instrumental Analysis, China Pharmaceutical University, Nanfing 210009, China; 4. Key Labo- ratory of Drug Quality Control and Pharmucovigilance Affiliated to Ministry of Education, China Pharma- ceutical University, Nanjing 210009, China) |
| Abstract: | Objective： To establish a method to evaluate the quality of Poria peel. Methods： A simple gradient RP-HPLC program was carried out to determine the content of poricoie acid A and 3-epidehydro- tumulosie acid simultaneously. A Dikma Dimonsal C18 column （250mm×4.6mm,5μm） was used, the mobile phase was 0.1% formic acid-acetonitrile, at a flow rate of 1 mL·min^-1, with the column tempera-ture of 25℃, and the detection wavelength was 243 nm. Results： The linear range of poricoie acid A and 3-epidehydrotumulosic acid was 0.073-0.292 g·L^-1（ r=0.9997） and 0.027-0.135 g·L^-1（ r = 0.9999）, respectively. And the recoveries of the two components were 102.63% and 98.88%, respec-tively. Conclusion： The method is rapid and accurate to determine the content of poricoic acid A and 3-epidehydrotumulosic acid in Poria peel. It＇s an easy way which also provides the good detection means for the quality control of Poria peel. |
| Keywords: | Poria peel poricoic acid A 3-epidehydrotumulosic acid RP-HPLC |
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