活性炭吸附分离芳香族氨基酸的研究

采用动态吸附法,对18种活性炭进行了筛选,并确定吸附条件为pH3,吸附温度25℃,炭-液比1∶12,吸附时间120min。根据活性炭吸附前后的蛋清寡肽混合物溶液的全波长紫外扫面结果,发现活性炭对芳香族氨基酸吸附作用较强。经氨基酸自动分析仪测得蛋清高F值寡肽溶液F值为22,游离氨基酸含量7.8%,凝胶过滤色谱测定其分子质量分布为连续的,绝大部分分布在300～800u。

Study on the Absorption and Desorption of Aromatic Amino Acids with Active Carbon

The absorption of eighteen active carbon was investigated. The optimal active carbon was determined and its absorption was studied. The optimal conditions were: No.4 active carbon, used to remove free aromatic amino acids by the method of static absorption; the weight of activated carbon to the volume of hydrolysate ratio 1∶12; the temperature was 25℃, pH3; the time was 120min. The result of UV-scanning indicated that active carbon was efficient to absorb aromatic amino acids. The amino acids composition of the Fischer ratio of egg white oligo-peptides was studied. G-25 gel filtration chromatography was used to determine the molecular weight of the oligo-peptied. The molecular weight ranged within 300~800.