| 紫外分光光度法测定山楂中的总黄酮含量 |
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| 引用本文： | 姜玉梅,王潇蕤. 紫外分光光度法测定山楂中的总黄酮含量[J]. 广东化工, 2009, 36(4): 183-186 |
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| 作者姓名： | 姜玉梅 王潇蕤 |
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| 作者单位： | 湖南化工职业技术学院,湖南,株洲,412004;湖南化工职业技术学院,湖南,株洲,412004 |
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| 摘 要： | 在0．008-0.040mg／mL线性范围内，建立以硝酸铝-亚硝酸钠为显色剂，紫外分光光度法测定山楂总黄酮含量方法。结果表明，该法简便易行、快速、准确。并对山楂总黄酮的乙醇提取工艺进行了研究，优选出最佳山楂提取工艺为乙醇浓度为60％，回流提取2次，每次1．5-2h。
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| 关 键 词： | 总黄酮 紫外分光光度法 含量测定 乙醇提取 |
UV Spectrophotometric Determination of Total Hawthorn Flavonoids |
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| Jiang Yumei,Wang Xiaorui. UV Spectrophotometric Determination of Total Hawthorn Flavonoids[J]. Guangdong Chemical Industry, 2009, 36(4): 183-186 |
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| Authors: | Jiang Yumei Wang Xiaorui |
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| Affiliation: | (Hunan Chemical Industry and Vocational Technology College, Zhuzhou 412004, China) |
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| Abstract: | Within 0.008-0.040 mg/mL linear range, the author aimed to set up an analytical method of UV spectrophotometric determination of total flavonoids in hawthorn, using aluminum nitrate sodium nitrite as chromogenic reagent. The results showed that the method was simple, rapid and accurate. The ethanol extraction process of the total flavonoids in hawthorn was also studied, optimizing for the best extraction process of hawthorn ethanol concentration was 60 % with reflux extraction twice, 1.5-2 h each time. |
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| Keywords: | total flavonoids UV spectrophotometry determinati on ethanol extract |
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