羟基丁酸-羟基辛酸聚合物/胶原软骨组织工程支架的细胞亲和性 |
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作者姓名: | 陆 兴 张永红 李二峰 王增荣 赵良启 |
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作者单位: | 1山西医科大学第二医院骨科,山西省太原市 030001
2山西大学生物技术研究所,山西省太原市 030006 |
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基金项目: | 国家自然科学基金(主任基金)(31040030) |
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摘 要: | 背景:已有很多实验证明,单独高分子材料或生物性材料制备的组织工程支架无法满足组织工程研究。
目的:评价羟基丁酸-羟基辛酸聚合物/胶原组织工程支架的生物学特性及细胞亲和性。
方法:以羟基丁酸-羟基辛酸聚合物作为主体材料,按质量分数复合不同比例(2%,4%,6%,8%,10%)的胶原,采用溶剂浇铸-颗粒沥滤法制备组织工程支架。通过扫描电镜观察材料内部结构及孔径大小,液体位移法测定材料孔隙率。将羟基丁酸-羟基辛酸聚合物/胶原支架、羟基丁酸-羟基辛酸聚合物支架分别与兔软骨细胞复合培养,MTT法测定细胞的生长曲线,扫描电镜观察细胞在材料上的生长黏附情况。
结果与结论:羟基丁酸-羟基辛酸聚合物/胶原复合软骨组织工程支架孔径大小200 μm左右,孔隙率为(85±2)%,细胞亲水性随加入胶原比例的增加而升高。与羟基丁酸-羟基辛酸聚合物支架比较,不同比例的羟基丁酸-羟基辛酸聚合物/胶原支架可明显促进软骨细胞的黏附、增殖。证实羟基丁酸-羟基辛酸聚合物/胶原复合支架具备更好的细胞亲和性。中国组织工程研究杂志出版内容重点:生物材料;骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性;组织工程全文链接:
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关 键 词: | 生物材料 软骨生物材料 羟基丁酸-羟基辛酸聚合物(PHBHOx) 胶原 软骨 组织工程 国家自然科学基金 |
Cell affinity of cartilage tissue engineering scaffolds prepared by poly(hydroxybutyrate-co-hydroxyoctanoate)/collagen composite materials |
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Authors: | Lu Xing Zhang Yong-hong Li Er-feng Wang Zeng-rong Zhao Liang-qi |
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Affiliation: | 1Department of Orthopedics, the Second Hospital of Shanxi Medical University, Taiyuan 030001, Shanxi Province, China
2Institute of Biotechnology, Shanxi University, Taiyuan 030006, Shanxi Province, China |
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Abstract: | BACKGROUND:Many experiments have demonstrated that tissue engineering scaffolds prepared by polymer materials alone or biomaterials cannot meet the requirement of tissue engineering research.
OBJECTIVE:To evaluate biological characteristics and cell affinity of poly(hydroxybutyrate-co-hydroxyoctanoate)/collagen composite scaffold.
METHODS:Tissue engineering scaffolds were prepared by combination of poly(hydroxybutyrate-co-hydroxyoctanoate) and collagen at different proportions (2%, 4%, 6%, 8% and 10%) using solvent casting/particulate leaching method. Inner structure and apertures were observed by scanning electron microscope, and the porosity was determined by liquid displacement method. Rabbit chondrocytes were co-cultured with poly(hydroxybutyrate-co-hydroxyoctanoate)/collagen composite scaffold and poly(hydroxybutyrate-co-hydroxyoctanoate) scaffold. Growth curve of cells was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and cell adhesion on the scaffolds was observed by scanning electron microscope.
RESULTS AND CONCLUSION:The pore size and porosity of the composite scaffold were about 200 μm and (85±2)%, respectively. The cell affinity dynamically increased with the increasing of proportion of collagen. Compared with the poly(hydroxybutyrate-co-hydroxyoctanoate) scaffold, the poly(hydroxybutyrate-co- hydroxyoctanoate)/collagen composite scaffolds are better to improve cell adhesion and proliferation, with favorable cellular affinity. |
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Keywords: | biocompatible materials 3-hydroxybutyric acid collagen chondrocytes |
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