电离辐射对T淋巴细胞IL-2分泌和细胞毒活性的影响

正常人外周血单个核细胞（ＰＢＭＣ）体外经１～６Ｇｙγ射线照射后，间接免疫荧光法分析受照Ｔ细胞Ｔ细胞抗原受体（ＴＣＲ）、ＣＤ＿３及ＣＤ＿（２３）阳性细胞百分率，Ｈ￣３－ＴｄＲ掺入及释放法测定白细胞介素－２（ＩＬ－２）分泌及Ｔ细胞细胞毒活性，免疫细胞化学法分析Ｔ细胞内ＴＣＲ及ＣＤ＿３表达．结果表明，Ｔ细胞ＴＣＲ、ＣＤ．及ＣＤ＿（２５）表达及ＩＬ－２分泌和细胞毒活性皆呈照射剂量依赖性降低．受照Ｔ细胞ＩＬ－２分泌及细胞毒活性抑制在一定程度上与细胞表面ＴＣＲ、ＣＤ＿３及ＣＤ＿（２５）表达减少、活性损伤有关，而细胞表面ＴＣＲ和ＣＤ＿３表达降低可能与大量ＴＣＲ及ＣＤ＿３在受照细胞胞浆内堆积有关．

Effects of radiation on IL-2 secretion and cytotoxicity of T cells

Peripheral blood mononuclearcells (PBMC ) from normal personswere irradiated with 1-6 Gb arabs invitro' The method of indirect imtnunofluorescenee was used for analysisof positiye percentage of TCR, CD3and CD25 of T cells' The method ofall-TdR incorpration and release wasused for determination of IL~2 secretion and T cell cytotoxicity,and immunocytochemistry was used for analysis of expression of TCR and CD3'Adecreasing pattern of TCR, CD3 andCD25 expression, IL~2 secretion,and T cell cytotoxicity depending onradiation dose was found. The inhibition of Ic--2 secretion and cytotoxicityafter irradiation of T cells wasassociated, in some degreej withdecreasing f expression and damagingactivity of TCR, CD3 and CD25,whereas it was possible that thedecreasing expression of TCR andCD3 at the cell surface was relatedto accummulation of TCR and CD3in cytoplasma of irradiated T cells'