| 人血小板生成素（hTPO）的cDNA克隆与序列测定 |
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| 引用本文： | 邢桂春 胡志远. 人血小板生成素（hTPO）的cDNA克隆与序列测定[J]. 高技术通讯, 1996, 6(3): 12-14 |
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| 作者姓名： | 邢桂春 胡志远 |
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| 作者单位： | 北京放射医学研究所 |
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| 基金项目： | 国家自然科学基金,863计划资助 |
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| 摘 要： | 由于人血小板生成素ｃＤＮＡ的阅读框架较长而且序列中无合适的酶切位点，因此，我们利用一个同义突变创造的ＫｐｎＩ酶切位点人为将ｈＴＰＯ ｃＤＮＡ分成Ｎ－端和Ｃ－端两个片段。首先利用反转录聚合酶链式反应从人胎肝ｍＲＮＡ中分别扩增出两个ｃＤＮＡ片段，于ＥｃｏＲＩ，ＫｐｎＩ位点分别克隆入测序载体ｐＵＣ１９中。
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| 关 键 词： | cDNA克隆 序列分析 人血小板生成素 |
cDNA Cloning and Sequencing of Human Thrombopoietin |
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| Xing Guichun,Hu Zhiyuan,Yang Xiaoming,Xie Ling,He Fuchu,Wu Chutse. cDNA Cloning and Sequencing of Human Thrombopoietin[J]. High Technology Letters, 1996, 6(3): 12-14 |
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| Authors: | Xing Guichun Hu Zhiyuan Yang Xiaoming Xie Ling He Fuchu Wu Chutse |
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| Abstract: | A Synonymous Substitution was used to set up a novel restriction enzyme site-KpnI,separating the total cDNA into two fragments,encoding N terminal and C terminal,respectively.The fragments were amplified from human fetalliver mRNA,coined into pUC 19 and sequenced.Their sequences were shown to be identical with hTPO cDNA reported. |
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| Keywords: | Human thrombopoietin RT-PCR cDNA cloning Sequencing |
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