多器官功能不全综合征时肠道细菌易位及通里攻下法影响的实验研究

肠道是全身感染的起源，细菌易位在ＭＯＤＳ的发生发展中具有重要作用。本实验以放射性同位素３５Ｓ标记致病Ｅｃｏｌｉ作示踪剂，研究不同剂量酵母多糖腹腔注射所致ＭＯＤＳ时肠道细菌易位的途径和程度，并观察通里攻下中药和抗生素对细菌易位的影响。结果表明，酵母多糖腹腔注射能造成肠道屏障损伤引起细菌易位，细菌易位的程度具有剂量依赖性。细菌易位途径主要有两条，低剂量时细菌易位以肠系膜淋巴结途径为主，高剂量时以门静脉途径为主。予先应用新霉素和灭滴灵给肠道脱污染反而加重细菌易位，抗生素治疗对细菌移位效果不明显。以大承气汤为代表的通里攻下中药对控制细菌易位有显著效果

THE BACTERIA TRANSLOCATION IN MODS RATS AND INHIBITORY EFFECTS OF TONGLIGONGXIA HERBS

The gut is the origin of systemic infection and MODS. Bacterial translocation may play a major role in the development of sepsis and MODS. We have previousely reported the Zymosan A injected intraperitoneally could cause gut barrier failure and be related to MODS. To determine the route and degree of bacterial translocation and observe the effects of TongLiGongXia Herbs on bcterial tranclocation (BT) in the MODS induced by injection intraperitoneally with various dose of Zymosan, 48 adult SD rats were gavaged with 35 S radioisotope-labelled Escherichia coli 4 hours before they were killed. Radionuclied counts were measured in blood and heart, liver, spleen, kidney as well as MLN. The results demonstrate that: 1) radio-labelled bacteria could reach systemic organs via the portal blood and the mesenteric lymph from the gut, 2) the BT was dose-dependent to zymosan, at a zymosan dose of 0.1mg/g, the mesenteric lymph was the major route, whereas at a dose of 0.5mg/g or higher, the bacteria translocate to systemic organ via the portal blood, 3) pretreatment with Neomycin and Metronidazol could promote BT, antibiotic-Ampicilin could not inhibite BT, the TongLiGongXia herbs was able to inhibite the BT.