高效液相色谱法测定保健食品多维康胶囊中叶酸的含量

目的建立高效液相色谱法(high performance liquid chromatography,HPLC)测定多维康胶囊中叶酸含量的方法。方法采用C₁₈(150mm×4.6mm,5μm)色谱柱,以磷酸二氢钾-甲醇溶液为流动相,流速0.8 mL/min,检测波长254 nm,柱温30℃,进样体积10μL。结果叶酸的线性关系良好,相关系数为1.000;方法检出限为0.034μg/mL,定量限为0.091μg/mL;方法平均加标回收率为98.80%~104.17%,平均相对标准偏差为0.02%。结论该方法操作简单,灵敏度高,可作为保健食品多维康胶囊中叶酸的测定方法。

Determination of folic acid in health food Duoweikang capsule by high-performance liquid chromatography

Objective To establish a method for the determination of folic acid in Duoweikang capsules by high performance liquid chromatography(HPLC).Methods C18(150 mm×4.6 mm,5μm)column was used with potassium dihydrogen phosphate methanol solution as mobile phase,the flow rate was 0.8 mL/min,the detection wavelength was 254 nm,the column temperature was 30℃,and the injection volume was 10μL.Results The linear relationship of folic acid was good,the correlation coefficient was 1.000;the limit of detection was 0.034μg/mL,the limit of quantitation was 0.091μg/mL;the average recoveries were 98.80%-104.17%,and the average relative standard deviation was 0.02%.Conclusion The method is simple,sensitive and can be used for the determination of folic acid in Duoweikang capsules.