莪术油对大鼠乳腺癌癌前病变组织中C-erbB-2、P53表达的影响

目的:通过观察莪术油对大鼠乳腺癌癌前病变组织中C-erbB-2、P53的表达,探讨莪术油治疗乳腺癌癌前病变的机理。方法:275只SD大鼠随机分为空白对照组、疾病模型组、三苯氧胺组、康莱特组及莪术油小、中、大剂量组。采用DMBA诱导乳腺癌癌前病变造模,干预治疗4周,第8至14周分4批处死动物,进行病理学及C-erbB-2、P53免疫组化检测。结果:各试验组不同类型乳腺组织中C-erbB-2、P53表达阳性率呈递增趋势。非典型增生乳腺组织中,各干预组C-erbB-2、P53表达阳性率均明显低于造模对照组(P0.05),莪术油组低于康莱特组(P0.05)。结论:莪术油能够降低DMBA诱导大鼠乳腺癌癌前病变组织中癌基因的表达,可能是其阻断乳腺癌发生的有效机制。

Effect of Zedoray Turmeric Oil on Expressions of C-erbB-2 and P53 in Precancerous Lesion of Rats Mammary

Objective: To research the treatment mechanism of Zedoray Turmeric Oil(ZTO)in precancerous lesion of rats mammary by measuring the expressions of C-erbB-2 and P53.Methods:275 SD rats were randomly divided into 7 groups:the blank control group(BC),the precancerous lesion model control group(PL),the kanglaite control group(KLT),the tamoxifen control group(TAM),the low dose group of ZTO(ZTO-L),the middle dose group of ZTO(ZTO-M)and the high dose group of ZTO(ZTO-H).Animal model of precancerous lesion of rats mammary was induced by DMBA and was treated by ZTO,KLT and TAM for 4 weeks.All the animals were killed from 8th to 14th week once every two weeks.Examination of histopathology was performed the expressions of C-erbB-2 and P53 were measured by immunohistochemistry.Results:The positive rate of expressions of C-erbB-2 and P53 trend to increase in every groups except BC corresponding to normal,usual ductal hyperplasia(UDH),atypical ductal hyperplasia(ADH)and invasive carcinoma.In ADH,the positive rate of expressions of C-erbB-2 and P53 of ZTO,KLT and TAM was much lower than PL(P<0.05).The positive rate of expressions of C-erbB-2 and P53 of ZTO,KLT and TAM was much lower than PL(P<0.05),and the positive rate of expressions of C-erbB-2 and P53 of ZTO was much lower than KLT(P<0.05).Conclusion:Zedoray Turmeric Oil inhibit the oncogenes of breast epithelial tissue in precancerous lesion of rats mammary induced by DMBA,which could be the mechanism to prevent the breast cancer.