| 高效液相色谱法测定参苏口服液中橙皮苷的含量 |
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| 引用本文： | 骆勇,苌玲.高效液相色谱法测定参苏口服液中橙皮苷的含量[J].贵阳医学院学报,2012,37(1):71-73. |
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| 作者姓名： | 骆勇 苌玲 |
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| 作者单位： | 骆勇 (贵州省食品药品检验所,贵州贵阳,550004) ； 苌玲 (贵州省食品药品检验所,贵州贵阳,550004) ； |
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| 摘 要： | 目的:建立以高效液相色谱法(HPLC)测定参苏口服液中橙皮苷含量的方法。方法:采用Waters-symmetry C18(WAT054275,5μm,250 mm×4.6 mm)色谱柱,流动相为甲醇-醋酸-水(35∶4∶61),流速:1 ml/min,检测波长为283 nm。结果:橙皮苷在0.073 56～1.103 4μg浓度内,峰面积与浓度呈良好线性关系(r=0.999 9);加样回收率为96.91%～98.31%,RSD=0.53%(n=6)。结论:此方法简便、快速、准确、重复性好,可用于参苏口服液的质量控制。
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| 关 键 词： | 橙皮苷 陈皮 色谱法 高压液相 参苏口服液 |
Determination of Hesperidin in Shensu Oral Liquid with HighPerformance Liquid Chromatography |
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| LUO Yong,CHANG Ling.Determination of Hesperidin in Shensu Oral Liquid with HighPerformance Liquid Chromatography[J].Journal of Guiyang Medical College,2012,37(1):71-73. |
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| Authors: | LUO Yong CHANG Ling |
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| Affiliation: | (Institute for Food and Drug Quality Control of Guizhou Province,Guiyang 550004,Guizhou,China) |
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| Abstract: | Objective: To establish a method for determination of hesperidin in Shensu oral liquid with high performance liquid chromatography(HPLC).Methods: HPLC was carried out on Waters-symmetry C18(WAT054275,5 μm,250 mm×4.6 mm) column with methyl alcohol-acetic acid-water(35∶4∶61) as mobile phase,and the flow rate was 1 ml/min.Detecion wavelength was 283 nm.Results: The calibration curve for hesperidin was linear in the range of 0.073 56～1.103 4 μg(r=0.999 9).The average recovery was 96.91%～98.31%,and RSD% was 0.53%(n=6).Conclusions: This method is simple,quick,accurate and reproducible for quality control of Shensu oral liquid. |
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| Keywords: | hesperidin Pericapium citrt chromatography high pressure liquid shensu oral liquid |
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