胃癌组织中辅助性T细胞因子1水平与胃癌患者预后的关系

目的探讨辅助性T细胞（Th1）因子在胃癌组织中的表达与胃癌患者预后的相关性及其临床意义。方法回顾性分析2006年6月至2008年1月间复旦大学附属中山医院收治的58例胃癌患者的临床资料,运用实时定量PCR技术检测58例胃癌组织中Th1细胞因子TRAV10、IRF1、TBX21、CD3Z、GZMB、GNLY、GATA3和IFNG8种Th1细胞的mRNA表达水平,并分析其与58例胃癌患者术后生存的关系。结果58例胃癌患者中位随访期为42．5（1～64）个月。术后1年和3年生存率为84．5％和72．4％。单因素Cox回归分析显示,淋巴结转移、远处转移、TNM分期、脉管癌栓、GNLYmRNA表达水平及Th1细胞8因子综合表达水平与胃癌术后生存有关（均P〈0．05）。GNLY的mRNA表达水平上调者和下调者3年生存率分别86．2％和58．6％．Th1细胞8因子任意上调和全部下调者3年生存率分别为79．6％和33．3％。差异均有统计学意义（均P〈0．05）。多因素Cox回归分析显示．淋巴结转移和Thl细胞8因子综合表达水平是本组胃癌患者的独立预后因素（均P〈0．05）。结论在Th1细胞因子中．GNLYmRNA表达水平与胃癌患者预后有关．但并不是独立因素。Th1细胞8因子综合表达水平可作为胃癌患者的独立预后因素。

Association of T helper cell 1 cytokines expressions with prognosis of gastric cancer patients

Objective To investigate the association of the expressions of Thl cytokines in gastric cancer tissue and the prognosis of patients with gastric cancer after radical resection. Methods Fifty-eight patients with gastric cancer treated at the Zhongshan Hospital of Fudan University were retrospectively analyzed. The expressions of Thl cytokines mRNA were detected in tumor tissues by real-time polymerase chain reaction （RT-PCR） method in Thl cells including TRAV10, IRF1, TBX21, CD3Z, GZMB, GATA3, and IFNG. Association of Thl cytokines mRNA expressions and prognosis was evaluated. Results The median follow up was 42.5（1-64） months. The 1-year survival rate was 84.5% and the 3-year survival rate was 72.4%. Univariate Cox regression analysis showed that lymph node metastasis, distant metastasis, TNM staging, lymphovascular invasion, GNLY mRNA expression, and the overall expression level of the 8 types of Thl cells were associated with the prognosis of patients with gastric cancer（all P〈0.05）. The 3- year survival was 86.2% in patients with increased expression of mRNA and 58.6% in those with decreased expression. The 3-year survival was 79.6% in patients with any increase in the 8 Thl cytokines and 33.3% in those with consistent downregulation of the 8 cytokines. Mutlivariate Cox analysis showed that lymph node metastasis and the overall expression level of 8 Thl cytokines were independent risk factors associated with the prognosis of patients with gastric cancer in this cohort （both P〈0.05）. Conclusions The mRNA expression of GNLY is associated with the prognosis of patients with gastric cancer but is not an independent risk factor. The combination of mRNA expressions of the eight cytokines is an independent prognostic factor.