| α-亚麻酸特异性脂肪酶产生菌的筛选和诱变初探 |
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| 引用本文： | 魏决,付忠旭,申毓晗,于志强,柴雪,彭夙由. α-亚麻酸特异性脂肪酶产生菌的筛选和诱变初探[J]. 食品科学, 2006, 27(1): 105-107 |
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| 作者姓名： | 魏决 付忠旭 申毓晗 于志强 柴雪 彭夙由 |
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| 作者单位： | 成都大学生物工程系,四川,成都,610016 |
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| 摘 要： | 从含油的泥土中采集60份样品，通过平板检测法进行初筛，摇瓶复筛，并测定酶活。复筛菌接种于平板，由菌落直径和其水解圈直径的比筛选出诱变出发菌株，用紫外光诱变方法对菌株进行诱变，由此分离出了埘富含α-亚麻酸的紫苏油具有一定专一水解能力的菌株，通过紫外诱变，其酶活较野生菌株增加了52.3％。
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| 关 键 词： | α-亚麻酸 特异性脂肪酶 诱变 酶活 |
| 文章编号： | 1002-6630（2006）01-0105-03 |
| 收稿时间： | 2005-01-27 |
| 修稿时间： | 2005-01-27 |
Studies on Screening and Mutagenesis of Special Lipase Production Strains of α -linolenic Acid |
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| WEI Jue,FU Zhong-xu,SHEN Yu-han,YU Zhi-qiang,CHAI Xue,PENG Su-you. Studies on Screening and Mutagenesis of Special Lipase Production Strains of α -linolenic Acid[J]. Food Science, 2006, 27(1): 105-107 |
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| Authors: | WEI Jue FU Zhong-xu SHEN Yu-han YU Zhi-qiang CHAI Xue PENG Su-you |
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| Affiliation: | Department of Biology, Chengdu University, Chengdu 610016, China |
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| Abstract: | 60 samples were selected from oil dregs of oil refining plant, screened by plate and liquate medium and menstruated lipase activity, starting strain was obtained by compared diameters of colony and hydrolyzed transparency circle on screening plate. The strain were obtained by mutagenesis with UV, which has high and special hydrolyze to perilla oil with rich a-linolenic acid and its lipase activity was 52.3% higher than that original strain. |
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| Keywords: | &alpha -linolenic acid； special lipase； mutagenesis； lipase activity |
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