Surface Charge Density of Hetero-Fused Plant Protoplasts: an Electrophoretic Study |
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Authors: | Obi, Ichiro Kakutani, Tadaaki Imaizumi, Nobutoshi Ichikawa, Yoshiaki Senda, Mitsugi |
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Affiliation: | 1Department of Agricultural Chemistry, Faculty of Agriculture, Kyoto University Kyoto, 606 Japan 2Research Center for Cell and Tissue Culture, Faculty of Agriculture, Kyoto University Kyoto, 606 Japan |
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Abstract: | Electrophoretic mobilities of hetero-fused plant protoplasts,which were obtained by electrofusion of barley mesophyll cellprotoplasts and Rauwolfia serpentina cultured cell protoplasts,and those of the unfused parent protoplasts were measured invarious media of different pH values. At pH 5.2, the zeta potentialof the fused protoplasts was intermediate between those of thebarley and R. serpentina protoplasts and the average surfacecharge density of the fused protoplasts was closer to that ofR. serpentina than to that of barley. The distribution of thesurface charge density of fused protoplast obtained at pH 5.2is discussed in terms of the surface charge densities and thesizes of parent protoplasts. These results revealed that thesurface charge density of fused protoplasts was determined bythe surface charge densities and the ratio of the surface areasof the respective parent protoplasts. (Received December 28, 1989; Accepted August 10, 1990) |
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