芬戈莫德对神经干细胞和星形胶质细胞增殖及迁移的影响及其对小鼠脊髓损伤的保护机制

目的：探讨芬戈莫德（FTY720）对神经干细胞（NSCs）和星形胶质细胞迁移及增殖的影响，阐明FTY720对小鼠脊髓损伤（SCI）的保护作用及其机制。方法：体外培养NSCs和星形胶质细胞，每种细胞随机分为对照组（采用PBS进行处理）和实验组（采用0.1μmol·L^-1FTY720进行处理）。SCI小鼠随机分为对照组（口服PBS）和实验组（口服1 mg·kg^-1·d^-1 FTY720），每组10只。采用Transwell实验检测2组NSCs和星形胶质细胞的迁移细胞数，免疫荧光染色检测NSCs的迁移细胞数和增殖神经球数，HE染色检测2组小鼠SCI空洞面积，Basso Mouse Scale （BMS）评分评估小鼠运动能力恢复情况。结果：Transwell检测，与对照组比较，实验组NSCs迁移细胞数增多（P<0.05），星形胶质细胞迁移细胞数减少（P<0.05）。免疫荧光检测，与对照组比较，实验组NSCs增殖神经球数增多（P<0.05），实验组小鼠SCI震中星形胶质细胞数减少（P<0.05）。HE染色检测，与对照组比较，SCI空洞面积缩小（P<0.05）。BMS评分，与对照组比较，实验组小鼠BMS评分升高（P<0.05）。结论：FTY720可以促进NSCs增殖和迁移，抑制星形胶质细胞迁移，减少SCI后胶质疤痕形成，促进小鼠运动功能恢复。

Effect of fingolimod on proliferation and migration of neural stem cells and astrocytes and its protective mechanism against spinal cord injury in mice

Objective: To investigate the effects of fingolimod (FTY720) on the migration and proliferation of neural stem cells (NSCs) and astrocytes of the mice, and to elucidate the protective effect of FTY720 on spinal cord injury(SCI) of the mice and its mechanism.Methods: The NSCs and astrocytes were cultured in vitro and divided into control group(treated with PBS) and experiment group(treated with 0.1 μmol·L^-1 FTY720). The SCI mice were divided into control group (given PBS by oral,n=10) and expriment group (given 1 mg·kg^-1·d^-1 FTY720 by oral,n=10). The number of migration cells of NSCs and astrocytes in two groups was detected by Transwell experiment, and the number of migration cells and the number of proliferative neurospheres of NSCs were detected by immunofluorescence staining.The SCI void areas of the mice in two groups were detected by HE staining, and the dynamic recovery of motor function of the mice was assessed by Basso Mouse Scale(BMS) behavioral score.Results: The Transwell detection results showed that compared with control group, the number of migration cells of NSCs in expriment group was increased(P<0.05), and the number of migration cells of astrocytes in expriment group was decreased(P<0.05). The immunofluorescence detection results showed that compared with control group, the number of proliferative neurospheres of NSCs in expriment group was increased(P<0.05),and the number of astrocytes in damaged SCI in expriment group was decreased(P<0.05).The HE staining results showed that compared with control group, the SCI void area was decreased(P<0.05). The BMS score results showed that the BMS score of the mice in expriment group was higher than that in control group(P<0.05).Conclusion: FTY720 can promote the proliferation and migration of NSCs, inhibit the migration of astrocytes, decrease the formation of glial scar, and promote the motor function recovery of the mice after SCI.