姜黄素调控大鼠骨髓间充质干细胞的成骨分化

背景：姜黄素能明显降低破骨细胞的骨重吸收，诱导破骨细胞凋亡，抑制破骨细胞形成。 目的：观察不同浓度姜黄素对大鼠骨髓间充质干细胞成骨分化的影响。 方法：采用贴壁法分离培养大鼠骨髓间充质干细胞，传至第3代后采用成骨培养基进行成骨诱导，在诱导前3 d于诱导培养基中分别加入0(对照)，10，15 μmol/L姜黄素。 结果与结论：接种培养7 d，倒置显微镜下可见分散的骨髓间充质干细胞小集落，集落细胞呈放射状生长。随培养时间延长，细胞集落增大，细胞形态为长梭形。加入成骨培养基7 d后细胞形态逐渐由长梭形变为多角形，随成骨诱导时间延长形态变化更加明显。加入不同浓度姜黄素后骨髓间充质干细胞增殖无变化。姜黄素呈剂量依赖性促进骨髓间充质干细胞碱性磷酸酶活性及Runx-2、血红素单加氧酶1的表达。提示姜黄素能有效促进大鼠骨髓间充质干细胞早期成骨分化，其机制可能与提高干细胞血红素单加氧酶1的表达有关。

Effect of curcumin on osteogenic differentiation of rat bone marrow mesenchymal stem cells

BACKGROUND:Curcumin can significantly reduce the bone resorption of osteoblasts, inhibit osteoclastogenesis and induce apoptosis in ostebclasts. OBJECTIVE:To investigate the effects of different concentrations of curcumin on osteoblast differentiation of rat bone marrow mesenchymal stem cells. METHODS:Rat bone marrow mesenchymal stem cells were isolated by adherent method. After subcultured to the third generation, osteogenic induction was performed with osteogenic culture medium. 0 (control), 10 and 15 μmol/L curcumin was added into the osteogenic medium at 3 days before induction. RESULTS AND CONCLUSION:After seeded and cultured for 7 days, scattered small colonies of bone marrow mesenchymal stem cells could be seen under the inverted microscope, and the colonies were in radial growth. As the culture time prolonged, the cell colonies were increased and the morphology of the cells was spindle shaped. At 7 days after addition of osteoblast medium, cells changed from spindle-shaped to cuboid and were more apparent by days. After addition of different concentrations of curcumin, there was no difference in the proliferation of bone marrow mesenchymal stem cells. Curcumin could increase the activity of alkaline phosphatase in bone marrow mesenchymal stem cells and the expression of Runx-2 and heme monooxygenase 1 in a dose-dependent manner. These findings demonstrate that curcumin can promote osteogenic differentiation of rat bone marrow mesenchymal stem cells. The effect of curcumin on osteogenic differentiation of rat bone marrow mesenchymal stem cells is correlated with heme monooxygenase 1 expression.