羟基丁酸-羟基辛酸聚合物-胶原骨软骨支架的制备及应用

背景：关节软骨修复的关键是软骨和软骨下骨的整体修复,然而目前尚缺乏理想的一体化支架。 目的：制备聚羟基丁酸-羟基辛酸-胶原一体化支架,并分析其基本生物学特性。 方法：以聚羟基丁酸-羟基辛酸、Ⅰ型胶原为材料,通过溶剂浇铸-颗粒沥滤法制备聚羟基丁酸-羟基辛酸-胶原一体化支架,观察支架超微结构,支架孔径及孔与孔的连通情况;液体置换法测定支架孔隙率。将乳兔骨髓间充质干细胞接种于聚羟基丁酸-羟基辛酸-胶原一体化支架上,扫描电镜观察细胞在支架上的黏附状态,MTT法测定细胞在支架上的生长曲线。 结果与结论：一体化支架呈疏松多孔结构,软骨层孔径80-100 μm,骨层孔径200-220 μm,孔隙率(80.0±2.3)%。骨髓间充质干细胞在支架上黏附状态良好,增殖迅速。说明聚羟基丁酸-羟基辛酸-胶原骨软骨一体化支架具备适宜的孔隙结构和良好的生物亲和性。

Preparation and application of poly (hydroxybutyrate-co-hydroxyoctanoate)/collagen composite scaffold

BACKGROUND:The key for articular cartilage repair is the overall repair of cartilage and subchondral bone; however, there is still lack of an ideal integrated scaffold. OBJECTIVE:To prepare the poly (hydroxybutyrate-co-hydroxyoctanoate)/collagen composite scaffold and to analyze the basic biological characteristics. METHODS:The poly (hydroxybutyrate-co-hydroxyoctanoate)/collagen composite scaffold were made of poly (hydroxybutyrate-co-hydroxyoctanoate) and collagen Ⅰ, fabricated using the solvent casting-particulate leaching method. The ultrastructure, pore size and interpores of the scaffolds were observed by scanning electron microscope. The porosity was measured by liquid displacement method. Bone marrow mesenchymal stem cells were isolated, amplified, and then inoculated onto the composite scaffolds. The adherent condition was observed by scanning electron microscope. At last, the proliferation of the bone marrow mesenchymal stem cells on the scaffolds was examined using MTT method, and the growth curve was drawn. RESULTS AND CONCLUSION:The integrated scaffold showed the loose and porous structure, The pore diameter of the chondral section was 80-100 μm, the pore diameter of the bone section was 200-220 μm, and the porosity of the osteochondral scaffold was (80.0±2.3)%. The bone marrow mesenchymal stem cells were adherent on the scaffolds well, and the proliferation of the cells on the composite scaffold was good. The pore structure and biocompatibility of poly (hydroxybutyrate-co-hydroxyoctanoate)/collagen composite scaffold was desirable.