混合物脑肽干预对肝纤维化大鼠模型的影响

背景：混合物脑肽是从动物脑中提取的多肽混合物,含有神经生长因子成分。近年研究发现,神经生长因子与肝损伤密切相关。目的：使用自拟混合物脑肽观察对大鼠肝纤维化的影响。方法：利用四氯化碳诱导形成SD大鼠肝纤维化模型。将大鼠随机分成纤维化模型组、脑肽干预组和对照组。4,8周采集标本。通过活体解剖取门静脉血清采用全自动生化分析仪检测肝功能丙氨酸氨基转移酶、天冬氨酸氨基转移酶、总胆红素、白蛋白;采用放射免疫法检测血清肝纤维化指标透明质酸、层粘连蛋白、Ⅲ型前胶原;常规苏木精-伊红和网状纤维染色检测肝组织标本。结果与结论：大鼠肝纤维化模型成功建立。脑肽干预组纤维化程度较纤维化模型组显著减轻,但肝组织炎症和肝细胞脂肪变性反而较纤维化模型组更显著。初步判断脑肽能够阻断四氯化碳诱导的肝纤维化,可能与神经生长因子有关。

Effect of brain peptide in rat models of liver fibrosis

BACKGROUND:Brain peptide is a kind of peptide mixture extracted from animal brain tissues, containing nerve growth factors. In recent years, studies have shown that nerve growth factors are closely related to liver injury.OBJECTIVE:To study the effect of brain peptide on liver fibrosis in rats.METHODS:Liver fibrosis was induced in rats by subcutaneous injection of CCl4 in SD rats, and then rat models were randomly divided into fibrosis model group, brain peptide intervention group and control group. The samples were collected at 4 and 8 weeks. Automatic biochemical analyzer was used to detect serum alanine aminotransferase, aspartate aminotransferase, total bilirubin, albumin. Radioimmunoassay detection of liver fibrosis markers, hyaluronic acid, laminin, procollagen III, was performed. Liver tissue samples were detected by hematoxylin-eosin and reticular fiber staining.RESULTS AND CONCLUSION:The rat liver fibrosis model was successfully established. Liver fibrosis was relieved significantly in the brain peptide intervention group than the fibrosis model group, but the extent of hepatic inflammation and fatty degeneration significantly increased. Brain peptide can effectually prevent CCl4-induced liver fibrosis, and the mechanism of preventing liver fibrosis may be related with nerve growth factors.