应用SRY原位杂交技术检测肝脏干细胞移植肝内种植情况

背景：以往干细胞种植后的定植示踪技术有磁探针标记、荧光示踪标记法、慢病毒感染荧光标记法及性别交叉Y染色体原位杂交检测法，各有优缺点。 目的：应用SRY原位杂交技术观察受体来源肝脏干细胞(肝卵圆细胞)在移植肝内的定植情况。 方法：二袖套法建立大鼠原位肝移植模型。供体为雌性DA大鼠，受体为雌性Lewis大鼠，设受体大鼠60只，肝卵圆细胞为Lewis大鼠来源的雄性细胞，移植中供肝种植肝卵圆细胞悬液。应用SRY原位杂交染色技术观察受体来源的雄性肝卵圆细胞在雌性移植肝内的定植情况。 结果与结论：经门静脉和肝动脉注射种植的肝卵圆细胞及其分化细胞主要定植在移植肝内中央静脉及汇管区周围，应用SRY原位杂交染色技术可以有效观察到受体来源肝脏干细胞在移植肝内的定植情况。

Y-chromosome in situ hybridization technology for detection of transplanted hepatic stem cells in the liver graft

BACKGROUND:The colonization tracer technology after stem cells transplantation includes magnetic probe labeling, fluorescent tracer method, lentivirus infections fluorescent labeling and Y chromosome in situ hybridization technology, and each method has its own disadvantages and advantages. OBJECTIVE:To observe the transplantation conditions of hepatic stem cells (hepatic oval cells) in the liver graft by Y-chromosome in situ hybridization technology. METHODS:Rat orthotropic liver transplantation models were established by using Kamada two-cuff technique. Female DA rats served as donors, and 60 female Lewis rats were used as recipients. Hepatic oval cells was originated from male Lewis rats and implanted to the liver graft during liver transplantation. Y-chromosome in situ hybridization technology was used to detect hepatic stem cells transplantation conditions in the liver graft. RESULTS AND CONCLUSION:Hepatic oval cells and differentiated cells mainly distributed around central vein area and portal area in the liver graft. The transplantation conditions of recipient derived hepatic stem cells in the liver graft were effectively detected by Y-chromosome in situ hybridization technology.