茶多酚对食管癌Eca-109细胞生长的影响

目的:研究茶多酚对食管癌Eca-109细胞的生长抑制作用。方法:采用MTT法检测茶多酚对食管癌Eca-109细胞生长活性的抑制，BrdU掺入实验分析茶多酚对食管癌Eca-109细胞增殖指数的影响，流式细胞分析仪分析茶多酚对食管癌Eca-109细胞周期及细胞凋亡的影响。结果:MTT实验证实茶多酚对食管癌Eca-109细胞生长有抑制作用，且呈剂量-效应关系。BrdU掺入实验证实茶多酚可以降低食管癌Eca-109细胞的生长分数。流式细胞仪检测发现茶多酚可以阻滞食管癌Eca-109细胞的细胞周期，使其停滞于G0/G1期，并诱导食管癌Eca-109细胞凋亡。结论:茶多酚对食管癌Eca-109细胞有抑制作用，机制可能与降低食管癌Eca-109细胞的增殖指数、阻滞细胞及诱导细胞凋亡有关。

Influence of tea polyphenols on the growth of esophageal cancer Eca - 109 cell

Objective:To study the influence of tea polyphenols on the growth of esophageal cancer-Eca-109 cell.Methods:MTT assay was used to detect the growth inhibition of the esophageal cancer-Eca-109 cell treated with tea polyphenols.BrdU staining was used to detect the growth index,flow cytometry was used to analyze the cell cycle,flow cytometry was used to analyze the apoptosis.Results:MTT assay confirmed that tea polyphenols inhibited the cell growth of esophageal cancer-Eca-109 cell with the dose-effect relationship.BrdU staining confirmed that tea polyphenols reduced the growth index of the esophageal cancer-Eca-109 cell.Flow cytometry displayed that tea polyphenols blocked the cell cycle of the esophageal cancer-Eca-109 in G0/G1 phase.Flow cytometry displayed that tea polyphenols induced the apoptosis of the esophageal cancer-Eca-109 cell.Conclusion:Tea polyphenol can inhibit the growth of the esophageal cancer-Eca-109 cell by reducing the proliferation index，blocking cell cycle and inducing cell apoptosis.