| dC14可抑制小鼠SRS腹水瘤的发病和生长 |
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| 引用本文： | 单易非,高红阳,纪宏,李丽春,裘慕绥,陈健,王德宝. dC14可抑制小鼠SRS腹水瘤的发病和生长[J]. 复旦学报(医学版), 1992, 0(5) |
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| 作者姓名： | 单易非 高红阳 纪宏 李丽春 裘慕绥 陈健 王德宝 |
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| 作者单位： | 上海医科大学基础医学部生物物理学教研室(单易非，高红阳，纪宏，李丽春)，中国科学院上海生物化学研究所(裘慕绥，陈健)，中国科学院上海生物化学研究所(王德宝) |
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| 摘 要： | 作者曾经体外实验证明,均聚寡脱氧胞嘧啶核苷酸片段(dC14)有抗病毒活性,现继续研究其在体内的作用。不给dC14的对照组小鼠,接种腹水瘤细胞后:100％发病和死亡,潜伏期为6d,存活期11d;给每只小鼠dC14 1.3～3.2 mg,发病率和死亡率降低至20％,疾病潜伏期和存活期推迟。在未经dC14处理的种瘤细胞和淋巴组织与经处理的淋巴组织中,多聚酶链反应(PCR)法都检测到病毒核酸,而正常小鼠中则无此种病毒核酸顺序。研究结果提示,dC14对小鼠SRS腹水瘤的发病和生长有抑制作用。此种抑制作用可能是通过抑制病毒基因的表达来实现的。
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| 关 键 词： | 均聚寡脱氧胞嘧啶核苷酸片段 腹水瘤病毒 基因表达 多聚酶链反应 |
dC14 CAN INHIBIT THE INCEPTION AND GROWTH OF MOUSE SRS ASCITIC TUMOR |
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| Shan Yifei,Gao Hongyang,Ji Hong,Li Lichun,Qiu Musui,Chen Jian,Wang Debao. dC14 CAN INHIBIT THE INCEPTION AND GROWTH OF MOUSE SRS ASCITIC TUMOR[J]. Fudan University Journal of Medical Sciences, 1992, 0(5) |
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| Authors: | Shan Yifei Gao Hongyang Ji Hong Li Lichun Qiu Musui Chen Jian Wang Debao |
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| Abstract: | The antiretroviral action of d014 in vitro has been proven in previous experiments. This paper deals with the effect of d014 in vivo.Intraperitoneal inoculation of tumor cells into mice without dC14 treatment induced ascitic tumors after a latency of 6 days and death of the mice after 11 days. Both incidence and mortality of disease were 100%. In animals receiving d014 treatment, the incidence and mortality were less, latency and survival periods were longer. Viral genes could be detected in tumor cells and lymphocytic tissues in tumor cell inoculated animals, receiving dC14 treatment or not but could not be detected in normal animals. Studies suggest that the suppressive action of dC14 on the development of mouse SRS ascitic tumor may be through block gene expression of virus. |
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| Keywords: | homo-oligo-dC14 aseitio tumor virus gene expression polyinerase chain reaction (PCR) |
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