脑出血患者体液免疫功能变化及其临床意义

目的　观察脑出血患者体液免疫功能的变化及其临床意义。方法　 115例脑出血患者中少量出血 39例 ,中等量出血 37例 ,大量出血 39例。采用流式细胞仪检测患者静脉血 CD19~+ CD2 5 ~+ 、CD19~+CD2 5~ - B淋巴细胞百分比 ,采用散射比浊法测定免疫球蛋白 Ig A、Ig G、Ig M和补体 C3水平 ,同时观察不同脑出血量之间上述指标的变化及急性期、恢复期的动态变化。结果　脑出血患者 CD19~+ CD2 5~ + 、CD19~+CD2 5 ~- B淋巴细胞百分比及 Ig A、Ig G、Ig M、C3含量均较正常对照组增高 (P<0 .0 5或 P<0 .0 1)。随着脑出血量的增多 ,CD19+ CD2 5 +、CD19+ CD2 5 - B淋巴细胞百分比及 Ig G、C3亦均呈增高趋势 (P均 <0 .0 5 ) ;恢复期 CD19~+ CD2 5 ~+、CD19+ CD2 5~ - B淋巴细胞百分比较急性期均降低 (P<0 .0 1和 P<0 .0 5 )。结论　脑出血患者存在体液免疫功能被激活的现象 ,出血量越多 ,疾病越严重 ,体液免疫功能被激活的程度越明显。

Changes in humoral immunological function and their clinical significance in patients with cerebral hemorrhage

Objective To study the changes in humoral immunological function and their clinical significance in cerebral hemorrhage. Methods Among 115 cases of cerebral hemorrhage, there was low volume bleeding in 39 case, middle volume in 37 cases, and large volume in 39 cases. The ratios of CD19 +CD25 + and CD19 +CD25 - B lymphocytes were determined by flow cytometry, and the content of IgA, IgG, IgM, and complement C3 were determined with scattered turbidimetry. The changes in humoral immunological function were compared in patients with different degrees of hemorrhage, and their variation in acute and recovery stages of disease were respectively assessed. Results The ratios of CD19 +CD25 + and CD19 +CD25 - B lymphocyte, and the levels of IgA, IgG, IgM, and C3 were higher than those of controls (all P <0 05). The ratios of CD19 +CD25 + and CD19 +CD25 - B lymphocyte, and the contents of IgA, IgG,IgM and C3 were increased with the increase in amount of hemorrhage(all P <0 05). The ratios of CD19 +CD25 + and CD19 +CD25 - B lymphocytes in recovery stage were lower than those in acute stage (all P <0 05). Conclusion There are activations of humoral immunological functions in cerebral hemorrhage. The more abundant in hemorrhage and the severer in disease, the more prominant in the changes in humoral immunological functions.