异丙酚对大鼠中枢神经核团一氧化氮合酶表达的影响

目的 通过检测异丙酚静脉全麻诱导大鼠中枢一氧化氮合酶(nitric oxide synthase, NOS)的表达,探讨异丙酚的可能中枢作用位点.方法 采用NADPH-d酶组织化学法检测大鼠脑片的NOS阳性神经元在神经核团的分布.结果 异丙酚作用的大鼠伏核、外侧隔核、下丘脑室旁核、膝状体腹下核、背外侧膝状核、视上核、视交叉上核、视前腹侧核、弧束核、杏仁基底外侧核、丘脑室旁核、外侧缰核、海马回嗅觉小岛及乳头状核等核团NOS阳性神经元的表达明显减少,与对照组比较均非常显著差异(P＜0.01).结论 异丙酚在大鼠中枢神经系统的作用部位与神经核团有关.

Nucleus distribution of nitric oxide synthase in central nervous system in rat after anesthetization with propofol

Objective To clarify the effective location of propofol in central nervous system (CNS). Methods Forty-two Wistar rats were ramdomized into control group,50 mg/kg propofol,100 mg/kg propofol,150 mg/kg propofol,tail shearing,propofol followed by tail shearing (n=7 in each group). The NOS expressions in the CNS were recorded by NADPH-d histochemistry after anesthesia by intraperitoneal injection of propofol. Results Rather widely stained NOS positive neurons were observed in the control group. In propofol groups,the NOS expressions were decreased significantly as compared with the control group,mainly located in ACB,LS,Pe,VLG,Den,SO,SCh,AVPO,Sol,SuM,BL,PV,LHb and Icj,showing a negative dose-effect relation with propofol. Conclusion Propofol has the determined sites of action in CNS and the decrease of NO synthesis by the inhibition of NOS may play a role in propofol-induced general anesthesia.