P53结合蛋白1在宫颈鳞癌中的表达及其临床意义

目的：探讨P53结合蛋白1（53BPI）在宫颈鳞癌组织中的表达情况及其与临床分期、分化程度、淋巴结转移以及病灶大小的关系。方法：选取2010年5月至2011年8月期间在温州医科大学附属第一医院妇科收治的临床资料完整的52例宫颈鳞癌、30例宫颈上皮内瘤变（CIN）和20例正常宫颈组织，采用实时荧光定量PCR技术检测53BP1 mRNA的表达水平，并对其表达水平与临床病理特征的关系进行统计学分析。结果：宫颈鳞癌组织中53BP1 mRNA相对表达水平明显低于正常组织，差异具有统计学意义（P〈0．05）。宫颈鳞癌中高分化组中53BP1 mRNA相对表达水平明显高于宫颈鳞癌低分化组，差异具有统计学意义（P〈0．05）。宫颈鳞癌无淋巴结转移组中53BP1 mRNA相对表达水平明显高于宫颈鳞癌淋巴结转移组，差异具有统计学意义（P〈0．05）。宫颈鳞癌FIGO I期组和FIGO Ⅱ期组以及宫颈鳞癌大病灶组和小病灶组间53BP1 mRNA相对表达水平比较，差异均无统计学意义（P〉0．05）。结论：53BP1在宫颈鳞癌组织中表达减低，与分化程度、淋巴结转移有关，与病灶大小、临床分期无明显相关。

Study on the expression and its clinical significance of 53BP1 in cervical carcinoma

Objective： To explore the expression of P53-binding protein 1 （53BP1） in the tissue of cervicalsquamous carcinoma and relationship among their clinical stages, the degree of differentiation, lesion of lymph node metastasis and size. Methods： Fifty-two cases of cervical squamous carcinoma, 30 cases of cervical intraepithelial neoplasia （CIN） and 20 cases of normal cervical tissue were selected. Then the real-time fluorescent quantitative PCR was adopted for the detection of 53BP1 mRNA expression, and the statistical analysis of its expression and its clinical pathological characteristics were complemented as well. Results： 53BP1 mRNA in cervical squamous carcinoma relative expression levels were significantly lower than that in normal tissue, and the difference was statistically significant （P〈0.05）. 53BP1 mRNA expression in the differentiation of cervical squa- mous carcinoma in higher group level was significantly higher than that in the group of poorly differentiated carcinoma of the cervix, and the difference was statistically significant （P〈0.05）. 53BP1 mRNA in cervical lymph node metastasis-free group relative expression levels were significantly higher than that in the cervical lymph node metastasis group, and the differences were statistically significant （P〈0.05）. Compared the expression levels in FIGO I group of cervical cancer and cervical carcinoma FIGO Ⅱ group, and focus-group and small lesions 53BP1 mRNA, the difference was not statistically significant （P〉0.05）. Conclusion： 53BP1 expression in cervical squamous cancer reduction is associated with the degree of differentiation, lymphatic metastasis, and lesion size, while it is not significantly related in clinical stages.