过量氟化物导致大鼠腰椎黄韧带退变与骨化

[目的]研究氟化物在黄韧带骨化中可能的作用机理。[方法]36只雄性SD大鼠，实验组饮用含NaF（质量分数为1014）蒸馏水，分别于3个月及6个月时，检测骨密度，血清、骨组织标本中Ca、P^3＋、Mg、Zn、Cu、Fe、F^-含量和血清碱性磷酸酶（ALP）活性；行大鼠腰段标本X线检查后组织病理观察。[结果]3个月实验组10只大鼠中6只出现氟斑牙：6个月实验组均出现氟斑牙，且大鼠中轴骨骨密度显著增加（P〈0．05）。X线检查，6个月实验组中有4只可见黄韧带嵴状骨化影。病理观察，3个月实验组大鼠黄韧带呈退行性改变；6个月实验组部分黄韧带骨化，骨化类型以膜内骨化为主。[结论]过量氟化物可造成SD大鼠腰椎黄韧带的退变、骨化，在黄韧带的骨化中可能起重要作用。

Effects of fluoride on the degeneration and ossification of the ligamentum flavum in rats

[Objective]To study the effects of fluoride on the degeneration and ossification of the ligamentum flavum(OLF).[Method]Thirty-six SD rats were divided into two groups,the experimental group was feeded with water of 100 ppm fluoride in distill water,and the control group with distill water.At the end of three and six months,the rats were analyzed with BMD,and the serum and illiac bone samples were collected for detecting the contents of Calcium,Phosphorus,Magnesium,Zinc,Cuprum,Ferrum and Fluoride.At last,the whole lumbar spine were taken for X-ray and pathological observation.[Result]The results showed dental fluorosis appeared in all objectives as compared to almost normal in control group.BMD of the lumbar spine was also greatly high in six month experimental group.The X-ray analysis showed that there were OLF(OLF appeared)in four rats in six month experimental group.Pathological observations showed in six month group,the changes of the ligamentum flavum were more severe than that in 3 months,and there was OLF oringnated from the attachments,mainly intramembranous ossification.[Conclusion]High content of fluoride could lead to the degeneration and ossification of the ligamnetum flavum in rat's lumbar spine,and might play some important role in the ossification of the ligamentum flavum.