清胰消积方对实验性胰腺癌体内生长和细胞周期的影响

目的:研究中药清胰消积方对人胰腺癌细胞株SW1990的体内抑瘤作用及其对细胞周期的影响。方法:荷瘤裸小鼠随机分为对照组、5-FU组、中药不同剂量组,治疗后计算抑瘤率,以流式细胞术检测肿瘤的细胞周期分布。结果:中药小、中、大剂量组抑瘤率分别为21.31%、38.16%及29.09%;细胞增殖指数为15.33%、15.78%及15.74%,均小于对照组(P<0.05)。中药组的G0/G1期细胞比例高于对照组,G2/M低于对照组(P<0.05)。结论:清胰消积中药对人胰腺癌体内生长有抑制作用,其机理之一是阻止肿瘤细胞进入增殖周期。

Impact of QINGYIXIAOJI Formula on Proliferation and Cell Cycle of Experimental Pancreatic Cancer in Vivo

Objective:To explore the effect of the traditional Chinese medicine Qingyi Xiaoji Formula(QYXJ) on the proliferation and cell cycles of human pancreatic cancer cell line SW1990 in vivo.Methods:Tumor burdened nude mice were randomized into control group,5-Fu group,and QYXJ groups at different doses.After treatment,inhibiting rates of tumor growth were calculated.The ratio of the cell cycles was measured by flow cytometry.Results:Inhibiting rate of tumor growth in the QYXJ groups was 12.31%,38.16% and 29.09%.Proliferation Index was 15.33%,15.78% and 15.74% respectively,significantly lower than that in the control group(P<0.05).The proportion of cells in G_0/G_1 phase was larger,and that in G_2/M phase was smaller for the QYXJ groups than that of the control group(P<0.05).Conclusion:QYXJ decotion may inhibit the proliferation of pancreatic cancer in vivo,possibly by blocking the cancer cells entering the proliferative phase.