| 用PCR法检测胸液中结核杆菌DNA |
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| 引用本文： | 赵君庸,曹美菊,李和平,雷金娥. 用PCR法检测胸液中结核杆菌DNA[J]. 西安交通大学学报(医学版), 1994, 0(1) |
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| 作者姓名： | 赵君庸 曹美菊 李和平 雷金娥 |
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| 摘 要： | 利用聚合酶链反应（ＰＣＲ）技术检测了３２例结核性渗出性胸膜炎和２０例非结核性疾病患者胸液中结核杆菌的６５ＫＤａ抗原蛋白编码基因的特异ＤＮＡ片段。结果表明，其检出率为７８．１３％，并具高度特异性。提示ＰＣＲ扩增ＤＮＡ技术对结核性渗出性胸膜炎是高度敏感和特异的早期、快速诊断方法。
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| 关 键 词： | 结核性渗出性胸膜炎；聚合酶链反应（PCR）；诊断；结核杆菌 |
DETECTION OF MYCOBACTERIUM TUBERCULOSIS DNA IN PLEURAL EFFUSION BY POLYMERASE CHAIN REACTION (PCR) |
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| Zhao Junyong, Cao Meiju, Li Heping, et al. DETECTION OF MYCOBACTERIUM TUBERCULOSIS DNA IN PLEURAL EFFUSION BY POLYMERASE CHAIN REACTION (PCR)[J]. Journal of Xi‘an Jiaotong University:Medical Sciences, 1994, 0(1) |
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| Authors: | Zhao Junyong Cao Meiju Li Heping et al |
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| Affiliation: | Department of Biochemistry |
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| Abstract: | The Special DNA fragment of coding gene of 65KDa antigen protein of mycobacterium tuberculosis in pleural effusion of 32 cases of tuberculous exudative pleurisy and 20 cases of nontuberculous disease were detected by PCR. The results showed that the positive rate of our PCR system was 78.13% and the specificity of detection by PCR was higher. Therefore,the paper suggests PCR amplification DNA is a highly sensitive and specific method for diagnosing tuberculous exudative pleurisy early and rapidly. |
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| Keywords: | tuberculous exudative pleurisy polymerase chain reaction diagnosis mycobacterium tuberculosis |
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