| 贝壳砂改良土壤中反硝化细菌的分析 |
| |
| 引用本文： | 张小远,肖瑶,田浩,王芸,刘彬彬,Asa Frostegar,赵立平,张晓君.贝壳砂改良土壤中反硝化细菌的分析[J].微生物学通报,2014,41(1):35-42. |
| |
| 作者姓名： | 张小远 肖瑶 田浩 王芸 刘彬彬 Asa Frostegar 赵立平 张晓君 |
| |
| 作者单位： | 1. 上海交通大学 生命科学技术学院 微生物代谢国家重点实验室 上海 200240;1. 上海交通大学 生命科学技术学院 微生物代谢国家重点实验室 上海 200240;1. 上海交通大学 生命科学技术学院 微生物代谢国家重点实验室 上海 200240;1. 上海交通大学 生命科学技术学院 微生物代谢国家重点实验室 上海 200240;2. 挪威生命科学大学 化学、生物技术及食品科学系 奥斯 N-1432 挪威;2. 挪威生命科学大学 化学、生物技术及食品科学系 奥斯 N-1432 挪威;1. 上海交通大学 生命科学技术学院 微生物代谢国家重点实验室 上海 200240;1. 上海交通大学 生命科学技术学院 微生物代谢国家重点实验室 上海 200240 |
| |
| 基金项目： | 国家自然科学基金项目(No. 41230856，21177086)；上海市国际合作项目(No. 12230706800) |
| |
| 摘 要： | 【目的】通过对一处经过长期使用贝壳砂进行改良的土壤中的反硝化细菌的多样性和细菌分离分析,研究该土壤中反硝化细菌的组成特征。【方法】采用454焦磷酸测序的方法分析了土壤样品中微生物群落的组成,选用Giltay培养基培养、鉴定从土壤中挑选的分离物的反硝化能力,并对具有反硝化能力的微生物进行了16S rRNA基因鉴定。【结果】该土壤样品中占据优势地位的为Proteobacteria、Acidobacteria、Bacteroidetes、Chloroflexi等门的微生物,属的水平上则有近70%尚未确立分类地位。所分离的细菌中,共得到12株厌氧条件下具有较高硝酸盐去除效率的微生物,分属Pseudomonas、Aeromonas、Serratia和Acinetobacter,均为γ变形菌纲的微生物。【结论】该土壤中具有较高的微生物多样性,包括很多未知类型的微生物和众多类型的反硝化细菌;分离到了11株具有反硝化能力的菌株,可用于该土壤的反硝化过程的进一步研究。
|
| 关 键 词： | 土壤，反硝化，高通量测序，群落，分离鉴定 |
Analysis of denitrifying bacteria in a long-term shell sand amended soil |
| |
| ZHANG Xiao-Yuan,XIAO Yao,TIAN Hao,WANG Yun,LIU Bin-Bin,ASA Frostegar,ZHAO Li-Ping and ZHANG Xiao-Jun.Analysis of denitrifying bacteria in a long-term shell sand amended soil[J].Microbiology,2014,41(1):35-42. |
| |
| Authors: | ZHANG Xiao-Yuan XIAO Yao TIAN Hao WANG Yun LIU Bin-Bin ASA Frostegar ZHAO Li-Ping and ZHANG Xiao-Jun |
| |
| Affiliation: | 1. State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;1. State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;1. State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;1. State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;2. Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Science, ?s N-1432, Norway;2. Department of Chemistry, Biotechnology and Food Science, Norwegian University of Life Science, ?s N-1432, Norway;1. State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China;1. State Key Laboratory of Microbial Metabolism, School of Life Science & Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China |
| |
| Abstract: | Objective] Analysis of the denitrifying bacteria composition in a long-term amended soil by pyrosequencing and isolating functional bacteria. Methods] Microbial community structure was analyzed by 454 pyrosequencing of 16S rRNA gene, Giltay medium was used to cultivate and screen denitrifying bacteria from the isolates obtained from the soil, 16S rRNA genes of the detected denitrifying bacteria were also identified. Results] Proteobacteria, Acidobacteria, Bacteroidetes, Chloroflexi were dominant in the soil sample and nearly 70% sequences were unclassified in genus level. Among 1 344 isolates, 12 isolates were found that could efficiently remove nitrate under anaerobic condition and were affiliated to Pseudomonas, Aeromonas, Serratia and Acinetobacter. Conclusion] The results showed a high microbial diversity in the soil and most of them were unclassified bacteria. Eleven strains of denitrifying bacteria were identified via nitrate removal and gas formation. These strains could be used for studying the denitrification property. |
| |
| Keywords: | Soil Denitrification High through-put sequencing Community Isolation |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《微生物学通报》浏览原始摘要信息 |
|
点击此处可从《微生物学通报》下载全文 |
|
