绿巨人组织培养及快速繁殖技术

目的]研究绿巨人的组织培养方法,建立其快速繁殖技术体系,为发展其产业化生产提供技术支撑。方法]以绿巨人嫩枝顶芽和腋芽为外植体,选择不同培养基和激素配比,进行诱导培养、继代培养、温室生根锻炼和大田移栽试验,初步建立绿巨人组织培养快速繁殖体系。结果]适宜的愈伤组织诱导培养基为：MS＋2.0 mg/L 6-BA＋0.05 mg/L NAA＋0.25 mg/L 2,4-D＋蔗糖30%;继代增殖培养基为：MS＋5.0 mg/L 6-BA＋0.02 mg/L NAA＋蔗糖30%;生根培养基为：MS＋0.5 mg/L IBA。在建立的快繁体系下辅以合适的外界条件,绿巨人炼苗移栽的成活率在95%以上。结论]研究为绿巨人组织培养及快速繁殖提供了技术支撑。

Research on the Tissue Culture of Spathiphyllum floribundum and Its Rapid Multiplication

Objective] The technical support for the industrialization development of Spathiphyllum floribundum was provided through the establishment of the rapid multiplication system based on the research on the method of its tissue culture.Method] The terminal bud and axillary bud of tender branch of Spathiphyllum floribundum were used as the explants and the different media and the different hormone compose in the medium were experimented in the selection of induction medium,subculture medium and rooting medium.And also,the adaptability of plantlet in greenhouse and its transplanting were experimented.So,the rapid multiplication system of Spathiphyllum floribundum was set up.Result] The results showed that the suitable medium for callus induction was MS+ 6-BA of 2.0 mg/L + NAA of 0.05 mg/L + 2,4-D of 0.25 mg/L +sucrose of 30%,the best subculture was MS+ 6-BA of 5.0 mg/L + NAA of 0.02 mg/L + sucrose of 30% and the best rooting medium was MS+IBA of 0.5 mg/L.Under the established rapid multiplication system and then supplemented by appropriate external conditions,the survival rate of the acclimatization transplanting of Spathiphyllum floribundum seedlings could reach above 95%.Conclusion]The research provided technical support of the tissue culture and rapid propagation of Spathiphyllum floribundum.